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Goat Anti-Rabbit IgG (H+L) Antibody (trFluor Europium Cryptate Conjugate) #86381

Filter:
  • ELISA+

    Product Specifications

    REACTIVITY Rab
    SENSITIVITY
    MW (kDa)
    Source/Isotype Goat
    Application Key:
    • ELISA+-ELISA and/or ELISA-like Assays 
    Species Cross-Reactivity Key:
    • Rab-Rabbit 

    Product Information

    Product Description

    This Cell Signaling Technology® antibody is the trFluor™ Europium Cryptate version of an affinity purified Goat Anti-Rabbit IgG (H+L) Antibody and is expected to exhibit rabbit polyclonal and monoclonal antibodies. Time-resolved (TR) fluorescence resonance energy transfer (FRET), or TR-FRET, data were generated using the conjugated antibody.

    Product Usage Information

    trFluor™ Europium Cryptate conjugated antibodies are ideal for protein-protein interaction assays, pair-based immunoassay technologies, or high-throughput screening where greater sensitivity and quantitative detection is needed. The long fluorescent decay time of Europium Cryptate results in improved signal to background over fluorescent dye conjugates. Methods utilizing Europium Cryptate conjugated antibodies include, but are not limited to, TR-FRET, where Europium Cryptate-labeled donor antibodies are paired with red fluorophore-labeled acceptor antibodies, such as Alexa Fluor® 647 or APC. Please contact us if you require an acceptor antibody clone conjugated to a red dye at a custom concentration, a carrier-free formulation, or a more customized packaging solution.

    Note: Addition of potassium fluoride (KF) in the assay dilution buffer range of 40-400 mM is recommended for enhanced fluorescent signal. Optimal dilutions and working concentrations of both the conjugated antibodies and the KF should be determined by the end user.

    Storage

    Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.

    Specificity / Sensitivity

    Goat Anti-Rabbit IgG (H+L) Antibody (trFluor™ Europium Cryptate Conjugate) recognizes native rabbit IgG.

    Species Reactivity:

    Rabbit

    Source / Purification

    The purified antibody was isolated from goat sera and prepared by affinity chromatography.

    Background

    Goat Anti-Rabbit IgG (H+L) Antibody is a secondary antibody designed to bind to primary antibodies generated from the rabbit species. It is used to detect and amplify signals in various assays.
      For Research Use Only. Not for Use in Diagnostic Procedures.
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