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IkappaB alpha (L35A5) Mouse Monoclonal Antibody (Amino-terminal Antigen) (Biotinylated) #7543

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  • WB

    Product Specifications

    REACTIVITY H M R Mk B Pg
    SENSITIVITY Endogenous
    MW (kDa) 39
    Source/Isotype Mouse IgG1
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 
    • B-Bovine 
    • Pg-Pig 

    Product Information

    Product Description

    This Cell Signaling Technology antibody is conjugated to biotin under optimal conditions. The biotinylated antibody is expected to exhibit the same species cross-reactivity as the unconjugated IκBα (L35A5) Mouse mAb (Amino-terminal Antigen) #4814.
    MW (kDa) 39

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 140 mM NaCl, 3 mM KCI, 10 mM sodium phosphate (pH 7.4) dibasic, 2 mM potassium phosphate monobasic, 2 mg/mL BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    IkappaB alpha (L35A5) Mouse Monoclonal Antibody (Amino-terminal Antigen) (Biotinylated) detects endogenous levels of total IκBα protein.

    Species Reactivity:

    Human, Mouse, Rat, Monkey, Bovine, Pig

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a GST-IκBα fusion protein corresponding to the amino terminus of human IκBα protein.

    Background

    The NF-κB/Rel transcription factors are present in the cytosol in an inactive state complexed with the inhibitory IκB proteins (1-3). Activation occurs via phosphorylation of IκBα at Ser32 and Ser36 followed by proteasome-mediated degradation that results in the release and nuclear translocation of active NF-κB (3-7). IκBα phosphorylation and resulting Rel-dependent transcription are activated by a highly diverse group of extracellular signals including inflammatory cytokines, growth factors, and chemokines. Kinases that phosphorylate IκB at these activating sites have been identified (8).

    Alternate Names

    EDAID2; I-kappa-B-alpha; IkappaBalpha; IkB-alpha; IKBA; MAD-3; MAD3; Major histocompatibility complex enhancer-binding protein MAD3; NF-kappa-B inhibitor alpha; NFKB inhibitor alpha; NFKBI; NFKBIA; nuclear factor of kappa light chain gene enhancer in B-cells; nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha

    For Research Use Only. Not for Use in Diagnostic Procedures.
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