Order 3, Get Your 4th Free!* | Learn More >>
4670
Loading Control Antibody Sampler Kit (HRP Conjugate)
Antibody Conjugates
Antibody Sampler Kit

Loading Control Antibody Sampler Kit (HRP Conjugate) #4670

Reviews ()
Citations (0)
Loading Control Antibody Sampler Kit (HRP Conjugate): Image 1

Western blot analysis of extracts from Jurkat, HeLa and COS cell lines, using COX IV (3E11) Rabbit mAb (HRP Conjugate).

Loading Control Antibody Sampler Kit (HRP Conjugate): Image 2

Western blot analysis of extracts from various cell lines using β-Tubulin (9F3) Rabbit mAb (HRP Conjugate).

Loading Control Antibody Sampler Kit (HRP Conjugate): Image 3

Western blot analysis of extracts from NIH/3T3 and HeLa cells using GAPDH (D16H11) XP® Rabbit mAb (HRP Conjugate).

Loading Control Antibody Sampler Kit (HRP Conjugate): Image 4

Western blot analysis of extracts from various cell lines using β-Actin (D6A8) Rabbit mAb (HRP Conjugate).

Loading Control Antibody Sampler Kit (HRP Conjugate): Image 5

Western blot analysis of extracts from various cell lines using Histone H3 (D1H2) XP® Rabbit mAb (HRP Conjugate).

To Purchase # 4670T
Product # Size Price
4670T
1 Kit  (5 x 20 µl) $ 391

Kit Usage Information

Protocols

Product Description

The Loading Control Antibody Sampler Kit (HRP Conjugate) contains antibodies directed against a variety of housekeeping proteins. These Cell Signaling Technology antibodies are conjugated to the carbohydrate groups of horseradish peroxidase (HRP) via its amine groups. The kit contains enough primary antibodies to perform two western blots.

Specificity / Sensitivity

Each antibody in the Loading Control Antibody Sampler Kit (HRP Conjugate) detects endogenous levels of its target protein and does not typically cross-react with other proteins. Histone H3 (D1H2) XP® Rabbit mAb (HRP Conjugate) detects endogenous levels of total histone H3 protein, including the Histone H3 variant CENP-A.

Source / Purification

Monoclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues near the amino terminus of human β-actin, surrounding Lys29 of human COX IV, near the carboxy terminus of human GAPDH, near the carboxy terminus of human histone H3, and near the amino terminus of human β-tubulin.

Background

Housekeeping proteins perform numerous basic functions within the cell and are constitutively expressed at high levels in a variety of tissues and cell types. Western blot analysis commonly uses housekeeping proteins such as β-actin, COX IV, GAPDH, histone H3 and the α- and β-tubulins as loading controls. Actin is a ubiquitous protein and a major component of the eukaryotic cytoskeleton. Actin exists mainly as the F-actin fibrous polymer (1). Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. Recent work has demonstrated that GAPDH plays roles in apoptosis (2), gene expression (3), and nuclear transport (4). Globular tubulin subunits made up of α- and β-tubulin heterodimers are the building blocks of microtubules, one of three types of cytosolic fibers that comprise the cytoskeleton (5). Histone proteins, including histone H3, make up the primary building block of chromatin known as nucleosomes. Modulation of the chromatin structure plays an important role in the regulation of transcription in eukaryotes (6). Cytochrome c oxidase (COX) is a hetero-oligomeric enzyme consisting of 13 subunits localized to the inner mitochondrial membrane (7-9). It is the terminal enzyme complex in the respiratory chain, catalyzing the reduction of protons across the mitochondrial inner membrane to drive ATP synthesis (10).

  1. Condeelis, J. (2001) Trends Cell Biol 11, 288-93.
  2. Hara, M.R. and Snyder, S.H. (2006) Cell Mol. Neurobiol. 26, 527-38.
  3. Zheng, L. et al. (2003) Cell 114, 255-66.
  4. Bae, B.I. et al. (2006) Proc. Natl. Acad. Sci. USA 103, 3405-9.
  5. Westermann, S. and Weber, K. (2003) Nat Rev Mol Cell Biol 4, 938-47.
  6. Workman, J.L. and Kingston, R.E. (1998) Annu Rev Biochem 67, 545-79.
  7. Ostermeier, C. et al. (1996) Curr. Opin. Struct. Biol. 6, 460-466.
  8. Capaldi, R.A. et al. (1983) Biochim. Biophys. Acta 726, 135-148.
  9. Kadenbach, B. et al. (2000) Free Radic. Biol. Med. 29, 211-221.
  10. Barrientos, A. et al. (2002) Gene 286, 53-63.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.