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MEK1 (61B12) Mouse Monoclonal Antibody (Biotinylated)

MEK1 (61B12) Mouse Monoclonal Antibody (Biotinylated) #5744

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Inquiry Info. # 5744

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Product Specifications

REACTIVITY	H M R Mk
SENSITIVITY	Endogenous
MW (kDa)	45
Source/Isotype	Mouse IgG2a

Application Key:

WB-Western Blotting

Species Cross-Reactivity Key:

H-Human
M-Mouse
R-Rat
Mk-Monkey

Product Information

Product Description

This Cell Signaling Technology antibody is conjugated to biotin under optimal conditions. The biotinylated antibody is expected to exhibit the same species cross-reactivity as the unconjugated MEK1 (61B12) Mouse mAb #2352.

MW (kDa)

Product Usage Information

Application	Dilution
Western Blotting	1:1000

Storage

Supplied in 140 mM NaCl, 3 mM KCI, 10 mM sodium phosphate (pH 7.4) dibasic, 2 mM potassium phosphate monobasic, 2 mg/mL BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

MEK1 (61B12) Mouse Monoclonal Antibody (Biotinylated) recognizes endogenous levels of total MEK1 protein. This antibody does not cross-react with MEK2 and other MAP kinase kinases.

Species Reactivity:

Human, Mouse, Rat, Monkey

Source / Purification

Monoclonal antibody is produced by immunizing animals with full length MEK1 proteins.

Background

MEK1 and MEK2, also called MAPK or Erk kinases, are dual-specificity protein kinases that function in a mitogen activated protein kinase cascade controlling cell growth and differentiation (1-3). Activation of MEK1 and MEK2 occurs through phosphorylation of two serine residues at positions 217 and 221, located in the activation loop of subdomain VIII, by Raf-like molecules. MEK1/2 is activated by a wide variety of growth factors and cytokines and also by membrane depolarization and calcium influx (1-4). Constitutively active forms of MEK1/2 are sufficient for the transformation of NIH/3T3 cells or the differentiation of PC-12 cells (4). MEK activates p44 and p42 MAP kinase by phosphorylating both threonine and tyrosine residues at sites located within the activation loop of kinase subdomain VIII.

Alternate Names

CFC3; Dual specificity mitogen-activated protein kinase kinase 1; ERK activator kinase 1; MAP kinase kinase 1; MAP2K1; MAPK/ERK kinase 1; MAPKK 1; MAPKK1; MEK 1; MEK1; MEL; mitogen-activated protein kinase kinase 1; MKK1; MP2K1; PRKMK1; protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)

Database Links

UniProt ID: Q02750

Entrez-Gene Id: 5604

Limited Uses

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For Research Use Only. Not for Use in Diagnostic Procedures.

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