Flow cytometric analysis of HeLa cells using CD44 (156-3C11) Mouse mAb (Pacific Blue™ Conjugate) #8383 (green) compared to concentration matched Mouse (G3A1) mAb IgG1 Isotype Control (Pacific Blue™ Conjugate) (red).
|Source/Isotype||Mouse IgG1 kappa|
This Cell Signaling Technology antibody is conjugated to Pacific Blue™ fluorescent dye and tested in-house for flow cytometry analysis in human cells.
Note: This control antibody must be diluted to the same concentration (not dilution) as the specific antibody in analysis. See Directions on Use.Directions for Use: Important! This control antibody must be diluted to the same concentration (not dilution) as the specific antibody used for analysis. Higher background fluorescence may result if excessive amounts of rabbit IgG isotype control are used. Do not use this antibody at 1:50 dilution in the same way as recommended for other Pacific Blue conjugated antibodies.
Important! Dilute this control antibody to the same concentration (not dilution) as the specific antibody used for analysis. Higher background may result if excessive amounts of mouse IgG1 isotype control is used.
Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Protect from light. Do not freeze.
Mouse (G3A1) mAb IgG1 Isotype Control (Pacific Blue™) is not directed against any known antigen. It functions as an isotype control for mouse IgG1 monoclonal antibodies.
Isotype control antibodies are used to estimate the nonspecific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.