Flow cytometric analysis of live human peripheral blood mononuclear cells using CD11c (3.9) Mouse mAb (PE Conjugate) #56025 (solid line) compared to concentration-matched Mouse (MOPC-21) mAb IgG1 Isotype Control (PE Conjugate) (dashed line). Analysis was performed on cells in the monocyte gate.
This Cell Signaling Technology antibody is conjugated to PE under optimal conditions and tested in-house for direct flow cytometric analysis in human cells.
Note: This control antibody must be diluted to the same concentration (not dilution) as the specific antibody in analysis. See Directions on Use.
Supplied in 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2. Do not aliquot the antibody. Protect from light. Do not freeze.
The antibody is not directed against any known antigen. It functions as an isotype control for mouse monoclonal antibodies.
Isotype control antibodies are used to estimate the nonspecific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.