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79339
Mouse (MOPC-21) mAb IgG1 Isotype Control (PE-Cy7® Conjugate)
Antibody Conjugates

Mouse (MOPC-21) mAb IgG1 Isotype Control (PE-Cy7® Conjugate) #79339

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Flow cytometric analysis of live human peripheral blood mononuclear cells using CD14 (61D3) Mouse mAb (PE-Cy7® Conjugate) #44947 (solid line) compared to concentration-matched Mouse (MOPC-21) mAb IgG1 Isotype Control (PE-Cy7® Conjugate) (dashed line). Analysis was performed on cells in the monocyte gate.

To Purchase # 79339S
Product # Size Price
79339S
500 µl $ 193

Supporting Data

Concentration 200 ug/ml
SENSITIVITY Endogenous
Isotype Mouse IgG1

Product Description

This Cell Signaling Technology antibody is conjugated to PE-Cy7® under optimal conditions and tested in-house for direct flow cytometric analysis in human cells.

Product Usage Information

Note: This control antibody must be diluted to the same concentration (not dilution) as the specific antibody in analysis. See Directions on Use.

Important for Use: Important: Dilute this control antibody to the same concentration (not dilution) as the specific antibody used for analysis. Higher background fluorescence may result if excessive amounts of mouse IgG isotype control are used. Do not use this antibody at 1:50 or 1:20 dilution like other CST PE-Cy® conjugated antibodies.

Storage:

Supplied in 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2. This product is stable for 6 months when stored at 4ºC. Do not aliquot the antibody. Protect from light. Do not freeze.

Specificity / Sensitivity

The antibody is not directed against any known antigen. It functions as an isotype control for mouse monoclonal antibodies.

Background

Isotype control antibodies are used to estimate the nonspecific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
Cy and CyDye are registered trademarks of GE Healthcare.