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Myc-Tag (9B11) Mouse Monoclonal Antibody (HRP Conjugate) #2040

Filter:
  • WB

    Product Specifications

    REACTIVITY All
    SENSITIVITY Endogenous
    MW (kDa) 65
    Source/Isotype Mouse IgG2a
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • All-All Species Expected 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 140 mM NaCl, 3 mM KCI, 10 mM sodium phosphate (pH 7.4) dibasic, 2 mM potassium phosphate monobasic, 2 mg/mL BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Myc-Tag (9B11) Mouse Monoclonal Antibody (HRP Conjugate) detects exogenously expressed proteins containing the Myc epitope tag. This antibody conjugate recognizes the Myc tag fused to either the amino or carboxy terminus of targeted proteins in transfected cells. This antibody conjugate detects exogenously expressed Myc-tagged proteins in cells expressed under a CMV promoter. Expression under other promoters has not been evaluated. This antibody conjugate may cross-react with c-myc protein. This antibody conjugate may weakly cross-react with a protein of unknown origin ~90kDa.

    Species Reactivity:

    All Species Expected

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues 410-419 of human c-Myc (EQKLISEEDL). This antibody was conjugated to HRP under optimal conditions.

    Background

    Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.

    The Myc epitope tag is widely used to detect expression of recombinant proteins in bacteria, yeast, insect and mammalian cell systems (1).
    For Research Use Only. Not for Use in Diagnostic Procedures.
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