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Myc-Tag Antibody (Fluorescein Conjugate) #2277

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Inquiry Info. # 2277

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    Product Specifications

    REACTIVITY H R Mk
    SENSITIVITY
    MW (kDa)
    SOURCE Rabbit
    Application Key:
    • IF-Immunofluorescence 
    • F-Flow Cytometry 
    Species Cross-Reactivity Key:
    • H-Human 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Immunofluorescence (Immunocytochemistry) 1:1000
    Flow Cytometry (Fixed/Permeabilized) 1:200

    Storage

    Supplied in PBS, 100 ug/ml BSA and 0.1% sodium azide. Store at 4 C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Myc-Tag Antibody (Fluorescein Conjugate) detects Myc-tagged recombinant proteins and is a useful tool for subcellular protein localization. The antibody is conjugated to fluorescein via amino-terminal amines and the epsilon amino groups on lysine. Fluorescein absorbs visible blue light at wavelength 490 nm and emits visible green light at 520 nm.

    Species Reactivity:

    Human, Rat, Monkey

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues 410-419 of human c-Myc (EQKLISEEDL). Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.

    The Myc epitope tag is widely used to detect expression of recombinant proteins in bacteria, yeast, insect and mammalian cell systems (1).
    For Research Use Only. Not for Use in Diagnostic Procedures.
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