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Nanog (D2A3) Rabbit Monoclonal Antibody (PE Conjugate) #60356

Filter:
  • F

    Product Specifications

    REACTIVITY M
    SENSITIVITY Endogenous
    MW (kDa)
    Source/Isotype Rabbit IgG
    Application Key:
    • F-Flow Cytometry 
    Species Cross-Reactivity Key:
    • M-Mouse 

    Product Information

    Product Description

    This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometric analysis in mouse cells. This antibody is expected to exhibit the same species cross-reactivity as the unconjugated Nanog (D2A3) XP® Rabbit mAb #8822.

    Product Usage Information

    Application Dilution
    Flow Cytometry (Fixed/Permeabilized) 1:50

    Storage

    Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibodies. Protect from light. Do not freeze.

    Protocol

    Specificity / Sensitivity

    Nanog (D2A3) Rabbit Monoclonal Antibody (PE Conjugate) recognizes endogenous levels of total mouse nanog protein. This antibody conjugate is expected to recognize nanog 1a and 1b isoforms. This antibody conjugate does not cross-react with human nanog.

    Species Reactivity:

    Mouse

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of mouse nanog protein.

    Background

    Nanog is a homeodomain-containing transcription factor that is essential for the maintenance of pluripotency and self renewal in embryonic stem cells (1). Nanog expression is controlled by a network of factors including Sox2 and the key pluripotency regulator Oct-4 (1). Recent advances in somatic cell reprogramming have utilized viral expression of combinations of transcription factors including nanog, Oct-4, Sox2, KLF4, c-Myc, and LIN28 (2,3).

    Alternate Names

    2410002E02Rik; early embryo specific expression NK family; Early embryo specific expression NK-type homeobox protein; ecat; ecat4; ENK; ES cell-associated protein 4; Homeobox protein NANOG; homeobox transcription factor Nanog; Nanog; Nanog homeobox; Q5TM83; Q80Z64; Q80Z64-2

    For Research Use Only. Not for Use in Diagnostic Procedures.
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