Confocal immunofluorescent analysis of mouse cerebellum using NeuN (D4G4O) XP® Rabbit mAb (Alexa Fluor® 594 Conjugate) (red) and GFAP (GA5) Mouse mAb (Alexa Fluor® 488 Conjugate) #3655 (green). Sections are mounted with ProLong® Gold Antifade Reagent with DAPI #8961.
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This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 594 fluorescent dye and tested in-house for direct immunofluorescent analysis in mouse tissue. This antibody is expected to exhibit the same species cross-reactivity as the unconjugated NeuN (D4G4O) XP® Rabbit mAb #24307.
Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
Cover sections with 4% formaldehyde dilute in 1X PBS.
NOTE: Formaldehyde is toxic, use only in fume hood.
NOTE: All subsequent incubations should be carried out at room temperature unless otherwise noted in a humid light-tight box or covered dish/plate to prevent drying and fluorochrome fading.
posted November 2006
revised November 2013
Protocol Id: 222
NeuN (D4G4O) XP® Rabbit mAb (Alexa Fluor® 594 Conjugate) recognizes endogenous levels of total NeuN protein.Species Reactivity:
Human, Mouse, Rat
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human NeuN protein.
Neuronal nuclei (NeuN, Fox-3, RBFOX3) is a nuclear protein expressed in most post-mitotic neurons of the central and peripheral nervous systems. NeuN is not detected in Purkinje cells, sympathetic ganglion cells, Cajal-Retzius cells, INL retinal cells, inferior olivary, or dentate nucleus neurons (1). This neuronal protein was originally identified by immunoreactivity with a monoclonal antibody also called NeuN. Using MS-analysis, NeuN was later identified as the Fox-3 gene product. Fox-3 contains an RNA recognition motif and functions as a splicing regulator (2). Fox-3 regulates alternative splicing of NumB, promoting neuronal differentiation during development (3).
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XP is a registered trademark of Cell Signaling Technology, Inc.
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