Render Target: STATIC
Render Timestamp: 2024-12-13T12:18:58.630Z
Commit: 611277b6de3cd1bb065350b6ef8d63df412b7185
XML generation date: 2024-10-16 17:30:20.946
Product last modified at: 2024-12-06T16:30:08.652Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Pan-Keratin (C11) Mouse mAb (Alexa Fluor® 750 Conjugate) #91322

Filter:
  • IHC
  • F

    Supporting Data

    REACTIVITY H M R Mk
    SENSITIVITY Endogenous
    MW (kDa)
    Source/Isotype Mouse IgG1
    Application Key:
    • IHC-Immunohistochemistry 
    • F-Flow Cytometry 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Description

    This Cell Signaling Technology® antibody is conjugated to Alexa Fluor® 750 fluorescent dye under optimal conditions. This antibody conjugate is expected to exhibit the same species cross-reactivity as the unconjugated Pan-Keratin (C11) Mouse mAb #4545.

    Product Usage Information

    Application Dilution
    Immunohistochemistry (Paraffin) 1:50 - 1:200
    Flow Cytometry (Fixed/Permeabilized) 1:1600

    Storage

    Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.

    Protocol

    Specificity / Sensitivity

    Pan-Keratin (C11) Mouse mAb (Alexa Fluor® 750 Conjugate) detects endogenous levels of total keratin 4, 5, 6, 8, 10, 13, and 18. This antibody conjugate does not cross-react with other keratins.

    Species Reactivity:

    Human, Mouse, Rat, Monkey

    Source / Purification

    Monoclonal antibody (isotype: IgG1) is produced by immunizing a BALB/c mouse with a cytoskeleton preparation from A-431 cells.

    Background

    KHSRP, also known as KSRP, is a KH domain-containing AU-rich element (ARE) binding protein (1). It recruits degradation machinery and activates mRNA turnover (2). This protein was previously shown to function as a regulator for splicing (3). KHSRP associates with both the Drosha and Dicer multiprotein complexes (4), and controls the biogenesis of some microRNAs by binding to the terminal loops of these microRNA precursors (3). KHSRP is found in neural and non-neural cell types in both the nucleus and the cytoplasm (4).
    For Research Use Only. Not For Use In Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    Alexa Fluor is a registered trademark of Life Technologies Corporation.
    This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or [email protected].
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