Flow cytometric analysis of MCF7 cells using Pan Keratin (C11) Mouse mAb (PE Conjugate) (blue) compared to concentration matched Mouse (G3A1) mAb IgG1 Isotype Control (PE Conjugate) #6899 (red).
|REACTIVITY||H M R Mk|
This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Pan-Keratin (C11) Mouse mAb #4545.
Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibodies. Protect from light. Do not freeze.
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
NOTE: If using whole blood, lyse red blood cells and wash by centrifugation prior to fixation.
posted July 2009
revised June 2017
Protocol Id: 407
Pan-Keratin (C11) Mouse mAb (PE Conjugate) detects endogenous levels of total keratin 4, 5, 6, 8, 10, 13 and 18. The antibody does not cross-react with other keratins.Species Reactivity:
Human, Mouse, Rat, Monkey
Monoclonal antibody is produced by immunizing a BALB/c mouse with a cytoskeleton preparation from A-431 cells.
Keratins (cytokeratins) are intermediate filament proteins that are mainly expressed in epithelial cells. Keratin heterodimers composed of an acidic keratin (or type I keratin, keratins 9 to 23) and a basic keratin (or type II keratin, keratins 1 to 8) assemble to form filaments (1,2). Keratin isoforms demonstrate tissue- and differentiation-specific profiles that make them useful as research biomarkers (1). Research studies have shown that mutations in keratin genes are associated with skin disorders, liver and pancreatic diseases, and inflammatory intestinal diseases (3-6).
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