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Phospho-ATF-2 (Thr71) (11G2) Rabbit Monoclonal Antibody (PE Conjugate)

Phospho-ATF-2 (Thr71) (11G2) Rabbit Monoclonal Antibody (PE Conjugate) #13850

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Inquiry Info. # 13850

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Product Specifications

REACTIVITY	H
SENSITIVITY	Endogenous
MW (kDa)
Source/Isotype	Rabbit IgG

Application Key:

F-Flow Cytometry

Species Cross-Reactivity Key:

H-Human

Product Information

Product Description

This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-ATF-2 (Thr71) (11G2) Rabbit mAb #5112.

Product Usage Information

Application	Dilution
Flow Cytometry (Fixed/Permeabilized)	1:50

Storage

Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibodies. Protect from light. Do not freeze.

Protocol

Specificity / Sensitivity

Phospho-ATF-2 (Thr71) (11G2) Rabbit Monoclonal Antibody (PE Conjugate) detects endogenous levels of ATF-2 only when phosphorylated at Thr71. The unconjugated antibody does not cross-react with phosphorylated c-Jun, CREB, or other transcription factors, but it does recognize ATF-2 dually phosphorylated at Thr69/Thr71 and singly phosphorylated at Thr71 equally well.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr71 of human ATF-2 protein.

Background

The transcription factor ATF-2 (also called CRE-BP1) binds to both AP-1 and CRE DNA response elements and is a member of the ATF/CREB family of leucine zipper proteins (1). ATF-2 interacts with a variety of viral oncoproteins and cellular tumor suppressors and is a target of the SAPK/JNK and p38 MAP kinase signaling pathways (2-4). Various forms of cellular stress, including genotoxic agents, inflammatory cytokines, and UV irradiation, stimulate the transcriptional activity of ATF-2. Cellular stress activates ATF-2 by phosphorylation of Thr69 and Thr71 (2-4). Both SAPK and p38 MAPK have been shown to phosphorylate ATF-2 at these sites in vitro and in cells transfected with ATF-2. Mutations of these sites result in the loss of stress-induced transcription by ATF-2 (2-4). In addition, mutations at these sites reduce the ability of E1A and Rb to stimulate gene expression via ATF-2 (2).

Alternate Names

Activating transcription factor 2; activating transcription factor 2 splice variant ATF2-var2; ATF-2; ATF2; cAMP response element-binding protein CRE-BP1; cAMP responsive element binding protein 2, formerly; cAMP-dependent transcription factor ATF-2; cAMP-responsive element-binding protein 2; CRE-BP1; CREB-2; CREB2; CREBP1; Cyclic AMP-dependent transcription factor ATF-2; Cyclic AMP-responsive element-binding protein 2; HB16; histone acetyltransferase ATF2; MGC111558; TREB7

Database Links

UniProt ID: P15336

Entrez-Gene Id: 1386

Limited Uses

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For Research Use Only. Not for Use in Diagnostic Procedures.

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