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REACTIVITY SENSITIVITY MW (kDa) Isotype
Rabbit 

Product Usage Information

Storage: Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibodies. Protect from light. Do not freeze.

Specificity / Sensitivity

Phospho-Cyclin D1 (Thr286) (D29B3) XP® Rabbit mAb (PE Conjugate) detects endogenous levels of cyclin D1 only when phosphorylated at Thr286. This antibody does not cross-react with other cyclin D family members.


Species predicted to react based on 100% sequence homology: Monkey

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr286 of cyclin D1 protein.

Product Description

This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-Cyclin D1 (Thr286) (D29B3) XP® Rabbit mAb #3300.


Activity of the cyclin-dependent kinases CDK4 and CDK6 is regulated by T-loop phosphorylation, by the abundance of their cyclin partners (the D-type cyclins), and by association with CDK inhibitors of the Cip/Kip or INK family of proteins (1). The inactive ternary complex of cyclin D/CDK4 and p27 Kip1 requires extracellular mitogenic stimuli for the release and degradation of p27 concomitant with a rise in cyclin D levels to affect progression through the restriction point and Rb-dependent entry into S-phase (2). The active complex of cyclin D/CDK4 targets the retinoblastoma protein for phosphorylation, allowing the release of E2F transcription factors that activate G1/S-phase gene expression (3). Levels of cyclin D protein drop upon withdrawal of growth factors through downregulation of protein expression and phosphorylation-dependent degradation (4).


1.  Hirai, H. et al. (1995) Mol Cell Biol 15, 2672-81.

2.  Sherr, C.J. (1996) Science 274, 1672-7.

3.  Lukas, J. et al. (1996) Mol Cell Biol 16, 6917-25.

4.  Diehl, J.A. et al. (1997) Genes Dev 11, 957-72.


Entrez-Gene Id 595
Swiss-Prot Acc. P24385


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.

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Phospho-Cyclin D1 (Thr286) (D29B3) XP® Rabbit mAb (PE Conjugate)