Phospho-Cyclin D1 (Thr286) (D29B3) XP^® Rabbit mAb (PE Conjugate) #8497

Phospho-Cyclin D1 (Thr286) (D29B3) XP^® Rabbit mAb (PE Conjugate) detects endogenous levels of cyclin D1 only when phosphorylated at Thr286. This antibody does not cross-react with other cyclin D family members.

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr286 of cyclin D1 protein.

This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-Cyclin D1 (Thr286) (D29B3) XP^® Rabbit mAb #3300.

Activity of the cyclin-dependent kinases CDK4 and CDK6 is regulated by T-loop phosphorylation, by the abundance of their cyclin partners (the D-type cyclins), and by association with CDK inhibitors of the Cip/Kip or INK family of proteins (1). The inactive ternary complex of cyclin D/CDK4 and p27 Kip1 requires extracellular mitogenic stimuli for the release and degradation of p27 concomitant with a rise in cyclin D levels to affect progression through the restriction point and Rb-dependent entry into S-phase (2). The active complex of cyclin D/CDK4 targets the retinoblastoma protein for phosphorylation, allowing the release of E2F transcription factors that activate G1/S-phase gene expression (3). Levels of cyclin D protein drop upon withdrawal of growth factors through downregulation of protein expression and phosphorylation-dependent degradation (4).

1.  Hirai, H. et al. (1995) Mol Cell Biol 15, 2672-81.

2.  Sherr, C.J. (1996) Science 274, 1672-7.

3.  Lukas, J. et al. (1996) Mol Cell Biol 16, 6917-25.

4.  Diehl, J.A. et al. (1997) Genes Dev 11, 957-72.

• Data sheet (127KB)
• MSDS PDF