Phospho-Cyclin D1 (Thr286) (D29B3) XP^® Rabbit mAb (PE Conjugate) #8497

Product Description

This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-Cyclin D1 (Thr286) (D29B3) XP^® Rabbit mAb #3300.

Storage:

Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibodies. Protect from light. Do not freeze.

Specificity / Sensitivity

Phospho-Cyclin D1 (Thr286) (D29B3) XP^® Rabbit mAb (PE Conjugate) detects endogenous levels of cyclin D1 only when phosphorylated at Thr286. This antibody does not cross-react with other cyclin D family members.

Species predicted to react based on 100% sequence homology:

Monkey

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr286 of cyclin D1 protein.

Background

Activity of the cyclin-dependent kinases CDK4 and CDK6 is regulated by T-loop phosphorylation, by the abundance of their cyclin partners (the D-type cyclins), and by association with CDK inhibitors of the Cip/Kip or INK family of proteins (1). The inactive ternary complex of cyclin D/CDK4 and p27 Kip1 requires extracellular mitogenic stimuli for the release and degradation of p27 concomitant with a rise in cyclin D levels to affect progression through the restriction point and Rb-dependent entry into S-phase (2). The active complex of cyclin D/CDK4 targets the retinoblastoma protein for phosphorylation, allowing the release of E2F transcription factors that activate G1/S-phase gene expression (3). Levels of cyclin D protein drop upon withdrawal of growth factors through downregulation of protein expression and phosphorylation-dependent degradation (4).

1. Hirai, H. et al. (1995) Mol Cell Biol 15, 2672-81.
2. Sherr, C.J. (1996) Science 274, 1672-7.
3. Lukas, J. et al. (1996) Mol Cell Biol 16, 6917-25.
4. Diehl, J.A. et al. (1997) Genes Dev 11, 957-72.

Pathways & Proteins

Explore pathways + proteins related to this product.

Select Your Pathway/Protein G1/S Checkpoint PI3K/Akt Signaling Protein: P24385

Database Links

Swiss-Prot Acc.: P24385

Entrez-Gene Id: 595

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