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REACTIVITY SENSITIVITY MW (kDa) Isotype
Rabbit 

Product Usage Information

Storage: Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.

Specificity / Sensitivity

Phospho-HSP27 (Ser82) (D1H2F6) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) recognizes endogenous levels of HSP27 protein only when phosphorylated at Ser82.


Species predicted to react based on 100% sequence homology: Mouse, Rat, Hamster, Bovine, Dog, Horse

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser82 of human HSP27 protein.

Product Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 488 fluorescent dye and tested in-house for direct flow cytometry and immunofluorescent analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-HSP27 (Ser82) (D1H2F6) XP® Rabbit mAb #9709.


Heat shock protein (HSP) 27 is one of the small HSPs that are constitutively expressed at different levels in various cell types and tissues. Like other small HSPs, HSP27 is regulated at both the transcriptional and posttranslational levels (1). In response to stress, the HSP27 expression increases several-fold to confer cellular resistance to the adverse environmental change. HSP27 is phosphorylated at Ser15, Ser78, and Ser82 by MAPKAPK-2 as a result of the activation of the p38 MAP kinase pathway (2,3). Phosphorylation of HSP27 causes a change in its tertiary structure, which shifts from large homotypic multimers to dimers and monomers (4). It has been shown that phosphorylation and increased concentration of HSP27 modulates actin polymerization and reorganization (5,6).


1.  Arrigo, A.P. and Landry, J. (1994) Cold Spring Harbor Laboratory Press, NY, 335-373.

2.  Landry, J. et al. (1992) J. Biol. Chem. 267, 794-803.

3.  Rouse, J. et al. (1994) Cell 78, 1027-37.

4.  Rogalla, T. et al. (1999) J. Biol. Chem. 274, 18947-18956.

5.  Lavoie, J. et al. (1993) J. Biol. Chem. 268, 24210-24214.

6.  Rousseau, S. et al. (1997) Oncogene 15, 2169-2177.


Entrez-Gene Id 3315
Swiss-Prot Acc. P04792


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
Alexa Fluor is a registered trademark of Life Technologies Corporation.
DRAQ5 is a registered trademark of Biostatus Limited.
The Alexa Fluor dye antibody conjugates in this product are sold under license from Life Technologies Corporation for research use only, except for use in combination with DNA microarrays. The Alexa Fluor® dyes (except for Alexa Fluor® 430 dye) are covered by pending and issued patents. Alexa Fluor® is a registered trademark of Molecular Probes, Inc.

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Phospho-HSP27 (Ser82) (D1H2F6) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate)