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Phospho-p90RSK (Ser380) (D5D8) Rabbit Monoclonal Antibody (PE Conjugate) #13397

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    Product Specifications

    REACTIVITY H M R Mk
    SENSITIVITY Endogenous
    MW (kDa)
    Source/Isotype Rabbit IgG
    Application Key:
    • F-Flow Cytometry 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Description

    This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-p90RSK (Ser380) (D5D8) Rabbit mAb #12032.

    Product Usage Information

    Application Dilution
    Flow Cytometry (Fixed/Permeabilized) 1:50

    Storage

    Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibodies. Protect from light. Do not freeze.

    Protocol

    Specificity / Sensitivity

    Phospho-p90RSK (Ser380) (D5D8) Rabbit Monoclonal Antibody (PE Conjugate) recognizes endogenous levels of RSK1, RSK2, and RSK3 proteins only when phosphorylated at Ser380 (RSK1), Ser386 (RSK2), or Ser377 (RSK3).

    Species Reactivity:

    Human, Mouse, Rat, Monkey

    The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.

    Species predicted to react based on 100% sequence homology:

    Chicken, Xenopus, Zebrafish, Bovine, Dog, Pig, Horse

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser377 of human p90RSK3 protein.

    Background

    The 90 kDa ribosomal S6 kinases (RSK1-4) are a family of widely expressed Ser/Thr kinases characterized by two nonidentical, functional kinase domains (1) and a carboxy-terminal docking site for extracellular signal-regulated kinases (ERKs) (2). Several sites both within and outside of the RSK kinase domain, including Ser380, Thr359, Ser363, and Thr573, are important for kinase activation (3). RSK1-3 are activated via coordinated phosphorylation by MAPKs, autophosphorylation, and phosphoinositide-3-OH kinase (PI3K) in response to many growth factors, polypeptide hormones, and neurotransmitters (3).

    Alternate Names

    90 kDa ribosomal protein S6 kinase 1; 90 kDa ribosomal protein S6 kinase 2; 90 kDa ribosomal protein S6 kinase 3; CLS; dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1); epididymis secretory sperm binding protein; HU-1; HU-2; HU-3; Insulin-stimulated protein kinase 1; ISPK-1; ISPK1; KS6A1; KS6A2; KS6A3; MAP kinase-activated protein kinase 1a; MAP kinase-activated protein kinase 1b; MAP kinase-activated protein kinase 1c; MAPK-activated protein kinase 1a; MAPK-activated protein kinase 1b; MAPK-activated protein kinase 1c; MAPKAP kinase 1a; MAPKAP kinase 1b; MAPKAP kinase 1c; MAPKAPK-1a; MAPKAPK-1b; MAPKAPK-1c; MAPKAPK1; MAPKAPK1A; MAPKAPK1B; MAPKAPK1C; mental retardation, X-linked 19; mitogen-activated protein kinase-activated protein kinase 1C; MRX19; p90-RSK 1; p90-RSK 2; p90-RSK 3; p90-RSK2; p90-RSK3; p90RSK; p90RSK1; p90RSK2; p90RSK3; p90S6K; pp90RSK2; pp90RSK3; ribosomal protein S6 kinase A1; ribosomal protein S6 kinase A2; ribosomal protein S6 kinase A3; ribosomal protein S6 kinase alpha 1; ribosomal protein S6 kinase alpha 2; Ribosomal protein S6 kinase alpha-1; Ribosomal protein S6 kinase alpha-2; Ribosomal protein S6 kinase alpha-3; ribosomal protein S6 kinase, 90kDa, polypeptide 1; ribosomal protein S6 kinase, 90kDa, polypeptide 2; ribosomal protein S6 kinase, 90kDa, polypeptide 3; Ribosomal S6 kinase 1; Ribosomal S6 kinase 2; Ribosomal S6 kinase 3; RPS6KA1; RPS6KA2; RPS6KA3; RSK; RSK-1; RSK-2; RSK-3; RSK1; RSK2; RSK3; S6K-alpha; S6K-alpha 1; S6K-alpha-1; S6K-alpha-2; S6K-alpha-3; S6K-alpha2; S6K-alpha3

    For Research Use Only. Not for Use in Diagnostic Procedures.
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