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13349S 100 µl (50 tests) $299.00.0
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REACTIVITY SENSITIVITY MW (kDa) Isotype
H M Mk Endogenous Rabbit IgG

Flow cytometric analysis of Jurkat cells, treated with U0126 (10uM, 2hr) (blue) or TPA (200uM, 30 min; green) using Phospho-PLCγ1 (Ser1248) (D25A9) Rabbit mAb (PE Conjugate) (solid lines) or concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control (PE Conjugate) #5742 (dashed lines).

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Product Usage Information

Application Dilutions
Flow Cytometry 1:50

Storage: Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibodies. Protect from light. Do not freeze.

Specificity / Sensitivity

Phospho-PLCγ1 (Ser1248) (D25A9) Rabbit mAb (PE Conjugate) recognizes endogenous levels of PLCγ1 protein only when phosphorylated at Ser1248.


Species Reactivity: Human, Mouse, Monkey
Species predicted to react based on 100% sequence homology: Rat

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser1248 of human PLCγ1 protein.

Product Description

This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-PLCγ1 (Ser1248) (D25A9) Rabbit mAb #8713.


Phosphoinositide-specific phospholipase C (PLC) plays a significant role in transmembrane signaling. In response to extracellular stimuli such as hormones, growth factors, and neurotransmitters, PLC hydrolyzes phosphatidylinositol 4,5-bisphosphate (PIP2) to generate two secondary messengers: inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG) (1). At least four families of PLCs have been identified: PLCβ, PLCγ, PLCδ, and PLCε. Phosphorylation is one of the key mechanisms that regulate the activity of PLC. PLCγ is activated by both receptor and non-receptor tyrosine kinases (2). PLCγ forms a complex with EGF and PDGF receptors, which leads to the phosphorylation of PLCγ at Tyr771, 783, and 1248 (3). Phosphorylation by Syk at Tyr783 activates the enzymatic activity of PLCγ1 (4). PLCγ2 is engaged in antigen-dependent signaling in B cells and collagen-dependent signaling in platelets. Phosphorylation by Btk or Lck at Tyr753, 759, 1197, and 1217 is correlated with PLCγ2 activity (5,6).


1.  Singer, W.D. et al. (1997) Annu Rev Biochem 66, 475-509.

2.  Margolis, B. et al. (1989) Cell 57, 1101-7.

3.  Kim, H.K. et al. (1991) Cell 65, 435-41.

4.  Wang, Z. et al. (1998) Mol Cell Biol 18, 590-7.

5.  Watanabe, D. et al. (2001) J Biol Chem 276, 38595-601.

6.  Ozdener, F. et al. (2002) Mol Pharmacol 62, 672-9.


Entrez-Gene Id 5335
Swiss-Prot Acc. P19174


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

13349
Phospho-PLCγ1 (Ser1248) (D25A9) Rabbit mAb (PE Conjugate)