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Phospho-S6 Ribosomal Protein (Ser240/244) (61H9) Rabbit Monoclonal Antibody (Biotinylated)

Phospho-S6 Ribosomal Protein (Ser240/244) (61H9) Rabbit Monoclonal Antibody (Biotinylated) #4278

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Inquiry Info. # 4278

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Product Specifications

REACTIVITY	H M R Mk
SENSITIVITY	Endogenous
MW (kDa)	32
Source/Isotype	Rabbit IgG

Application Key:

WB-Western Blotting
F-Flow Cytometry

Species Cross-Reactivity Key:

H-Human
M-Mouse
R-Rat
Mk-Monkey

Product Information

Product Description

This Cell Signaling Technology (CST) antibody is conjugated to biotin under optimal conditions. The unconjugated Phospho-S6 Ribosomal Protein (Ser240/244) (61H9) Rabbit mAb #4838 reacts with human, mouse, rat and monkey phospho-S6 ribosomal protein (Ser240/244). CST expects that Phospho-S6 Ribosomal Protein (Ser240/244) (61H9) Rabbit mAb (Biotinylated) will also recognize phospho-S6 ribosomal protein (Ser240/244) in these species.

MW (kDa)

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Flow Cytometry (Fixed/Permeabilized)	1:100

Storage

Supplied in 140 mM NaCl, 3 mM KCI, 10 mM sodium phosphate (pH 7.4) dibasic, 2 mM potassium phosphate monobasic, 2 mg/mL BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Phospho-S6 Ribosomal Protein (Ser240/244) (61H9) Rabbit Monoclonal Antibody (Biotinylated) detects endogenous levels of ribosomal protein S6 only when phosphorylated at Ser240 and Ser244.

Species Reactivity:

Human, Mouse, Rat, Monkey

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser240 and Ser244 of human S6 ribosomal protein.

Background

One way that growth factors and mitogens effectively promote sustained cell growth and proliferation is by upregulating mRNA translation (1,2). Growth factors and mitogens induce the activation of p70 S6 kinase and the subsequent phosphorylation of S6 ribosomal protein. Phosphorylation of S6 ribosomal protein correlates with an increase in translation of mRNA transcripts that contain an oligopyrimidine tract in their 5' untranslated regions (2). These particular mRNA transcripts (5'TOP) encode proteins involved in cell cycle progression, as well as ribosomal proteins and elongation factors necessary for translation (2,3). Important S6 ribosomal protein phosphorylation sites include several residues (Ser235, Ser236, Ser240, and Ser244) located within a small, carboxy-terminal region of S6 protein (4,5).

Alternate Names

40S ribosomal protein S6; Phosphoprotein NP33; ribosomal protein S6; RPS6; RS6; S6; S6 ribosomal protein; Small ribosomal subunit protein eS6

Database Links

UniProt ID: P62753

Entrez-Gene Id: 6194

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not for Use in Diagnostic Procedures.

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