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Phospho-Stat6 (Tyr641) (5A4) Mouse Monoclonal Antibody #9366

Inquiry Info. # 9366

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    Product Specifications

    REACTIVITY
    SENSITIVITY
    MW (kDa) 110
    Source/Isotype Mouse IgG2b

    Product Information

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Specificity / Sensitivity

    Phospho-Stat6 (Tyr641) (5A4) Mouse Monoclonal Antibody detects endogenous levels of Stat6 only when phosphorylated at tyrosine 641. This antibody does not significantly cross-react with the analogous phospho-tyrosines of other Stat proteins.

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr641 of human Stat6.

    Background

    Upon activation by Janus kinases, Stat6 translocates to the nucleus where it regulates cytokine-induced gene expression. Stat6 is activated via phosphorylation at Tyr641 and is required for responsiveness to IL-4 and IL-13 (1-4). In addition, Stat6 is activated by IFN-α in B cells, where it forms transcriptionally active complexes with Stat2 and p48 (5,6). Protein phosphatase 2A is also involved in regulation of IL-4-mediated Stat6 signaling (7).

    Alternate Names

    D12S1644; IL-4 Stat; IL-4-STAT; Signal transducer and activator of transcription 6; signal transducer and activator of transcription 6, interleukin-4 induced; STAT, interleukin4-induced; STAT6; STAT6B; STAT6C; transcription factor IL-4 STAT

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