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PTPRJ/DEP-1 (F4G8K) Rabbit mAb (Alexa Fluor® 647 Conjugate) #91111

Filter:
  • F

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa)
    Source/Isotype Rabbit IgG
    Application Key:
    • F-Flow Cytometry 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Description

    This Cell Signaling Technology® antibody is conjugated to Alexa Fluor® 647 fluorescent dye under optimal conditions. This antibody conjugate is expected to exhibit the same species cross-reactivity as the unconjugated PTPRJ/DEP-1 (F4G8K) Rabbit mAb #28386.

    Product Usage Information

    Application Dilution
    Flow Cytometry (Fixed/Permeabilized) 1:50
    Flow Cytometry (Live) 1:50

    Storage

    Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.

    Protocol

    Specificity / Sensitivity

    PTPRJ/DEP-1 (F4G8K) Rabbit mAb (Alexa Fluor® 647 Conjugate) recognizes endogenous levels of total PTPRJ/DEP-1 protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with recombinant protein specific to a fragment of the extracellular domain of human PTPRJ/DEP-1 protein.

    Background

    Protein-tyrosine phosphatase receptor type J (PTPRJ), also known as density-enhanced phosphatase-1 (DEP-1), is a heavily glycosylated, type 1 transmembrane protein. The extracellular region of PTPRJ/DEP-1 contains multiple copies of the fibronectin type III motif, while its intracellular region harbors a single PTP catalytic domain (1). PTPRJ/DEP-1 is expressed at varying levels on multiple hematopoietic cell lineages and has been shown to modulate signaling downstream of the TCR complex (2,3). Research studies have suggested that PTPRJ/DEP-1 also plays a role in non-hematopoietic cell biology through its ability to inhibit cell growth in response to increasing cell density (4). Indeed, PTPRJ/DEP-1-dependent dephosphorylation of mitogenic molecules, such as c-Met and PDGFR-β, has been shown to attenuate signaling downstream of these RTKs (5,6). In many contexts, PTPRJ/DEP-1 functions as a tumor suppressor, and its expression is lost in multiple types of human cancers (7,8).
    For Research Use Only. Not For Use In Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    Alexa Fluor is a registered trademark of Life Technologies Corporation.
    This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or [email protected].
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