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4096
Rabbit (DA1E) mAb IgG XP® Isotype Control (Biotinylated)
Antibody Conjugates
Monoclonal Antibody

Rabbit (DA1E) mAb IgG XP® Isotype Control (Biotinylated) #4096

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Immunoprecipitation Image 1 - Rabbit (DA1E) mAb IgG XP® Isotype Control (Biotinylated)

Immunoprecipitation of extracts from NIH/3T3 cells treated with PDGF using Rabbit (DA1E) mAb IgG XP® Isotype Control (Biotinylated) (lane 1) and Phospho-Akt (Ser473) (193H12) Rabbit mAb (Biotinylated) #3637 (lane 2). Biotinylated antibodies were pulled down using Streptavidin (Bead Conjugate) #3419. Western blot analysis was performed using Phospho-Akt (Ser473) (587F11) Mouse mAb #4051.

To Purchase # 4096S
Product # Size Price
4096S
100 µl $ 136

Supporting Data

Concentration 500 ug/ml
SENSITIVITY Endogenous
Source/Isotype Rabbit IgG

Application Key:

  • W-Western
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • IF-Immunofluorescence
  • F-Flow Cytometry
  • E-P-ELISA-Peptide

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • All-All Species Expected

Product Description

Rabbit (DA1E) mAb IgG XP® Isotype Control (Biotinylated) antibody was conjugated to biotin under optimal conditions and tested in-house for Immunoprecipitation.

Product Usage Information

Note: This control antibody must be diluted to the same concentration (not dilution) as the specific antibody in analysis. See Directions on Use.

Storage

Supplied in 136 mM NaCl, 2.6 mM KCI, 12 mM sodium phosphate (pH 7.4) dibasic, 2 mg/ml BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibodies.

Specificity / Sensitivity

Rabbit (DA1E) mAb IgG XP® Isotype Control (Biotinylated) is not directed against any known antigen. It functions as an isotype control for rabbit IgG monoclonal antibodies.

Background

Isotype control antibodies are used to estimate the nonspecific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.