Immunoprecipitation of extracts from NIH/3T3 cells treated with PDGF using Rabbit (DA1E) mAb IgG XP® Isotype Control (Biotinylated) (lane 1) and Phospho-Akt (Ser473) (193H12) Rabbit mAb (Biotinylated) #3637 (lane 2). Biotinylated antibodies were pulled down using Streptavidin (Bead Conjugate) #3419. Western blot analysis was performed using Phospho-Akt (Ser473) (587F11) Mouse mAb #4051.
Rabbit (DA1E) mAb IgG XP® Isotype Control (Biotinylated) antibody was conjugated to biotin under optimal conditions and tested in-house for Immunoprecipitation.
Note: This control antibody must be diluted to the same concentration (not dilution) as the specific antibody in analysis. See Directions on Use.
Supplied in 136 mM NaCl, 2.6 mM KCI, 12 mM sodium phosphate (pH 7.4) dibasic, 2 mg/ml BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibodies.
Rabbit (DA1E) mAb IgG XP® Isotype Control (Biotinylated) is not directed against any known antigen. It functions as an isotype control for rabbit IgG monoclonal antibodies.
Isotype control antibodies are used to estimate the nonspecific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.