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4096
Rabbit (DA1E) mAb IgG XP® Isotype Control (Biotinylated)

Rabbit (DA1E) mAb IgG XP® Isotype Control (Biotinylated) #4096

APPLICATIONS

Concentration SENSITIVITY Isotype
0.5 mg/ml Endogenous Rabbit IgG
IP

Immunoprecipitation of extracts from NIH/3T3 cells treated with PDGF using Rabbit (DA1E) mAb IgG XP® Isotype Control (Biotinylated) (lane 1) and Phospho-Akt (Ser473) (193H12) Rabbit mAb (Biotinylated) #3637 (lane 2). Biotinylated antibodies were pulled down using Streptavidin (Bead Conjugate) #3419. Western blot analysis was performed using Phospho-Akt (Ser473) (587F11) Mouse mAb #4051.

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Flow Cytometry

Flow cytometric analysis of HeLa cells using GAPDH (14C10) Rabbit mAb (Biotinylated) #5014 (blue) compared to Rabbit (DA1E) mAb IgG XP® Isotype Control (Biotinylated) (red).

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Note: This control antibody must be diluted to the same concentration (not dilution) as the specific antibody in analysis. See Directions on Use.

Storage:

Supplied in 136 mM NaCl, 2.6 mM KCI, 12 mM sodium phosphate (pH 7.4) dibasic, 2 mg/ml BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibodies.

Rabbit (DA1E) mAb IgG XP® Isotype Control (Biotinylated) is not directed against any known antigen. It functions as an isotype control for rabbit IgG monoclonal antibodies.

Rabbit (DA1E) mAb IgG XP® Isotype Control (Biotinylated) antibody was conjugated to biotin under optimal condtions and tested in-house for direct flow cytometry and western blot.

Isotype control antibodies are used to estimate the nonspecific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.

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