Render Target: STATIC
Render Timestamp: 2025-02-12T11:32:10.934Z
Commit: 7500bcdc731e9059bbdfbdbe9e72caa896e426e8
XML generation date: 2024-09-20 06:16:44.390
Product last modified at: 2025-01-01T09:03:57.062Z
Cell Signaling Technology Logo
1% for the planet logo
PDP - Template Name: Polyclonal Antibody (Isotype Control)
PDP - Template ID: *******afc9daa

Rabbit IgG Isotype Control (Alexa Fluor® 488 Conjugate) #4340

Filter:
  • F

    Supporting Data

    Concentration 100 µg/ml
    SENSITIVITY Endogenous
    Source/Isotype Rabbit
    Application Key:
    • F-Flow Cytometry 

    Product Information

    Product Description

    Rabbit IgG was conjugated to Alexa Fluor® 488 fluorescent dye under optimal conditions and tested in-house for direct flow cytometric analysis of human and mouse cells.

    Product Usage Information

    Note: This control antibody must be diluted to the same concentration (not dilution) as the specific antibody use in analysis. Higher background fluorescence may result if excessive amounts of isotype control are used. For protocol details, please reference the product page for the specific antibody used in analysis.

    Storage

    Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.

    Specificity / Sensitivity

    The purified antibody is not directed against any known antigen. It functions as an isotype control for rabbit antibodies.

    Source / Purification

    The antibody was isolated from naive rabbit sera and prepared by affinity chromotography. The antibody was conjugated to Alexa Fluor® 488 under optimal conditions with a F/P ratio of 2-6.

    Background

    Isotype control antibodies are used to estimate the nonspecific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.
      For Research Use Only. Not For Use In Diagnostic Procedures.
      Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
      This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or [email protected].
      All other trademarks are the property of their respective owners. Visit our Trademark Information page.