Flow cytometric analysis of Jurkat cells, using Rabbit IgG Isotype Control (Alexa Fluor® 488 Conjugate) (red) compared to Phospho-Akt (Ser473) (193H12) Rabbit mAb (Alexa Fluor® 488 Conjugate) #2336, untreated (green) or LY294002 and wortmannin treated (blue).
Flow cytometric analysis of Jurkat cells, using Rabbit IgG Isotype Control (Alexa Fluor® 488 Conjugate) (red) compared to Phospho-Stat3 (Tyr705) (D3A7) Rabbit mAb (Alexa Fluor® 488 Conjugate) #4323, untreated (blue) or IFN-α treated (green).
Rabbit IgG was conjugated to Alexa Fluor® 488 fluorescent dye under optimal conditions and tested in-house for direct flow cytometric analysis of human and mouse cells.
Note: This control antibody must be diluted to the same concentration (not dilution) as the specific antibody in analysis. See Directions on Use.Directions for Use: Important! This control antibody must be diluted to the same concentration (not dilution) as the specific antibody used for analysis. Higher background fluorescence may result if excessive amounts of rabbit IgG isotype control are used. Do not use this antibody at 1:50 dilution in the same way as recommended for other CST Alexa Fluor® conjugated antibodies.
Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
The purified antibody is not directed against any known antigen. It functions as an isotype control for rabbit antibodies.
The antibody was isolated from naive rabbit sera and prepared by affinity chromotography. The antibody was conjugated to Alexa Fluor® 488 under optimal conditions with a F/P ratio of 2-6.
Isotype control antibodies are used to estimate the nonspecific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
The Alexa Fluor dye antibody conjugates in this product are sold under license from Life Technologies Corporation for research use only, except for use in combination with DNA microarrays. The Alexa Fluor® dyes (except for Alexa Fluor® 430 dye) are covered by pending and issued patents. Alexa Fluor® is a registered trademark of Molecular Probes, Inc.