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63801
Rat (LTF-2) mAb IgG2b Isotype Control (APC-Cy7® Conjugate)
Antibody Conjugates

Rat (LTF-2) mAb IgG2b Isotype Control (APC-Cy7® Conjugate) #63801

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Flow cytometric analysis of live mouse splenocytes using MHC Class II (I-A/I-E) (M5/114.15.2) Rat mAb (APC-Cy7® Conjugate) #55413 (solid line) compared to concentration-matched Rat (LTF-2) mAb IgG2b Isotype Control (APC-Cy7® Conjugate) (dashed line).

To Purchase # 63801S
Product # Size Price
63801S
100 µg $ 169

Supporting Data

Concentration 200 ug/ml
SENSITIVITY Endogenous
Isotype Rat IgG2b

Product Description

This Cell Signaling Technology antibody is conjugated to APC-Cy7® under optimal conditions and tested in-house for direct flow cytometric analysis in mouse cells.

Product Usage Information

Note: This control antibody must be diluted to the same concentration (not dilution) as the specific antibody in analysis. See Directions for Use.

Directions for Use: Important: Dilute this control antibody to the same concentration (not dilution) as specific antibody in analysis. Higher background fluorescence may result if excessive amounts of rat IgG isotype control are used.

Storage:

Supplied in 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH 7.2. This product is stable for 6 months when stored at 4ºC. Do not aliquot the antibody. Protect from light. Do not freeze.

Specificity / Sensitivity

The antibody is not directed against any known antigen. It functions as an isotype control for rat monoclonal antibodies.

Background

Isotype control antibodies are used to estimate the nonspecific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
Cy and CyDye are registered trademarks of GE Healthcare.