RUNX2 (D1L7F) Rabbit mAb (PE Conjugate) #98059
- F
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Rabbit IgG |
Application Key:
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Description
Product Usage Information
| Application | Dilution |
|---|---|
| Flow Cytometry (Fixed/Permeabilized) | 1:50 |
Storage
Protocol
Specificity / Sensitivity
RUNX2 (D1L7F) Rabbit mAb (PE Conjugate) recognizes endogenous levels of total RUNX2 protein.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala273 of human RUNX2 protein.
Background
Runt-related transcription factor 2 (RUNX2) is a member of the RUNX family of transcription factors. It is involved in osteoblast differentiation and skeletal morphogenesis. RUNX2 regulates the transcription of various genes, including osteopontin, bone sialoprotein, and osteocalcin, via binding to the core site of the enhancers or promoters (1-3). RUNX2 is crucial for the maturation of osteoblasts and both intramembranous and endochondral ossification. Mutations in the corresponding RUNX2 gene have been associated with the bone development disorder cleidocranial dysplasia (CCD) (4-6). RUNX2 is also abnormally expressed in various human cancers, including prostate and breast cancer. It plays an important role in migration, invasion, and bone metastasis of prostate and breast cancer cells (7-10).
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- Willis, D.M. et al. (2002) J Biol Chem 277, 37280-91.
- Tu, Q. et al. (2008) J Cell Physiol 217, 40-7.
- Quack, I. et al. (1999) Am J Hum Genet 65, 1268-78.
- Cardoso, B.M. et al. (2010) Clin Dysmorphol 19, 150-2.
- Han, M.S. et al. (2010) J Cell Biochem 110, 97-103.
- Akech, J. et al. (2010) Oncogene 29, 811-21.
- van der Deen, M. et al. (2010) J Cell Biochem 109, 828-37.
- Barnes, G.L. et al. (2003) Cancer Res 63, 2631-7.
- Barnes, G.L. et al. (2004) Cancer Res 64, 4506-13.
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