Sox9 (D8G8H) Rabbit mAb (Biotinylated) #33636
- WB
Supporting Data
| REACTIVITY | H M |
| SENSITIVITY | Endogenous |
| MW (kDa) | 70 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
Product Information
Product Description
| MW (kDa) | 70 |
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Protocol
Specificity / Sensitivity
Sox9 (D8G9H) Rabbit mAb (Biotinylated) recognizes endogenous levels of total Sox9 protein.
Species Reactivity:
Human, Mouse
The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.
Species predicted to react based on 100% sequence homology:
Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human Sox9 protein.
Background
Sox9 is a transcription factor with an HMG-box DNA binding domain that has homology to the HMG domain of the mammalian testis-determining factor, SRY (1). Sox9 regulates several important processes during embryonic development including chondrogenesis, during which it contributes to skeletal formation and digit specification (2,3). Sox9 also coordinates with steroidogenic factor-1 to direct Sertoli cell-specific expression of anti-Mullerian hormone during embryogenesis, thereby contributing to male sex determination (4). In addition, Sox9 is reportedly involved in the maintenance of adult stem cell populations, including multipotent neural stem cells (5), hair follicle stem cells (6), and mammary stem cells (7). Recent interest has focused on the role of Sox9 in tumor biology. For example, research studies have shown that Sox9 expression in lung adenocarcinoma induces a mesenchymal phenotype in tumor cells (8). Other research studies have shown that YAP1 induced upregulation of Sox9 confers cancer stem cell like properties on esophageal cancer cells (9). Moreover, Sox9 expression has been linked with several other tumor types including ovarian, prostate, and pancreatic malignancies (10-12).
- Mertin, S. et al. (1999) Nucleic Acids Res 27, 1359-64.
- Akiyama, H. et al. (2002) Genes Dev 16, 2813-28.
- Raspopovic, J. et al. (2014) Science 345, 566-70.
- De Santa Barbara, P. et al. (1998) Mol Cell Biol 18, 6653-65.
- Scott, C.E. et al. (2010) Nat Neurosci 13, 1181-9.
- Kadaja, M. et al. (2014) Genes Dev 28, 328-41.
- Guo, W. et al. (2012) Cell 148, 1015-28.
- Capaccione, K.M. et al. (2014) Oncotarget 5, 3636-50.
- Song, S. et al. (2014) Cancer Res 74, 4170-82.
- Raspaglio, G. et al. (2014) Gene 542, 173-81.
- Qin, G.Q. et al. (2014) Onco Targets Ther 7, 137-46.
- Shroff, S. et al. (2014) Hum Pathol 45, 456-63.
Limited Uses
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