Stat5 (3H7) Rabbit mAb (Biotinylated)

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WB

Inquiry Info. # 4459

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Product Specifications

REACTIVITY	H
SENSITIVITY	Endogenous
MW (kDa)	90
Source/Isotype	Rabbit IgG

Application Key:

WB-Western Blotting

Species Cross-Reactivity Key:

H-Human

Product Information

Product Description

This Cell Signaling Technology antibody is conjugated to biotin under optimal conditions and tested in-house by western blot of human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated antibody (Stat5 (3H7) Rabbit mAb #9358).

MW (kDa)

90

Product Usage Information

Application	Dilution
Western Blotting	1:2000

Storage

Supplied in 140 mM NaCl, 3 mM KCI, 10 mM sodium phosphate (pH 7.4) dibasic, 2 mM potassium phosphate monobasic, 2 mg/mL BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Stat5 (3H7) Rabbit mAb (Biotinylated) detects endogenous levels of total Stat5a and b protein.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to a central region within human Stat5.

Background

Stat5 is activated in response to a wide variety of ligands, including IL-2, GM-CSF, growth hormone, and prolactin. Phosphorylation at Tyr694 is obligatory for Stat5 activation (1,2). This phosphorylation is mediated by Src upon erythropoietin stimulation (3). Stat5 is constitutively active in some leukemic cell types (4). Phosphorylated Stat5 is found in some endothelial cells treated with IL-3, which suggests its involvement in angiogenesis and cell motility (5). Stat5a and Stat5b are independently regulated and activated in various cell types. For instance, interferon treatment predominantly activates Stat5a in U-937 cells and Stat5b in HeLa cells (6).

Alternate Names

epididymis secretory sperm binding protein; GHISID2; MGF; Signal transducer and activator of transcription 5A; Signal transducer and activator of transcription 5B; STA5A; STA5B; STAT5; STAT5A; STAT5B; transcription factor STAT5B

Database Links

UniProt ID: P42229 , P51692

Entrez-Gene Id: 6776 , 6777

Limited Uses

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Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not for Use in Diagnostic Procedures.

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Stat5 (3H7) Rabbit mAb (Biotinylated) #4459