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SynGAP (E7J9K) Mouse mAb (Biotinylated)

SynGAP (E7J9K) Mouse mAb (Biotinylated) #64822

Supporting Data

REACTIVITY	H M R
SENSITIVITY	Endogenous
MW (kDa)	140
Source/Isotype	Mouse IgG2b kappa

Species Cross-Reactivity Key:

H-Human
M-Mouse
R-Rat

Product Information

Product Description

This Cell Signaling Technology^® antibody is the biotinylated version of the unconjugated SynGAP (E7J9K) Mouse mAb #62193 and is expected to exhibit the same species cross-reactivity. The concentration of the biotinylated antibody is 500 μg/mL. Peptide ELISA data were generated using the biotinylated antibody.

MW (kDa)

140

Product Usage Information

Biotinylated antibodies are ideal for immunoassay technologies and high-throughput ELISA platforms that require antibody pairs where both antibodies are from the same host. Platforms utilizing biotinylated antibodies include, but are not limited to, MSD, xMAP, Quanterix Simoa, AlphaLISA, AlphaScreen, HTRF, LANCE, and TR-FRET.

Optimal dilutions/working concentrations should be determined by the end user. Please contact us if you require the antibody clone biotinylated at a different concentration, a carrier-free formulation, or a more customized packaging solution.

Storage

Supplied in 140 mM NaCl, 3 mM KCI, 10 mM sodium phosphate (pH 7.4) dibasic, 2 mM potassium phosphate monobasic, 2 mg/mL BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

SynGAP (E7J9K) Mouse mAb (Biotinylated) recognizes endogenous levels of total SynGAP protein.

Species Reactivity:

Human, Mouse, Rat

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro1097 of human SynGAP protein.

Background

SynGAP is a synaptic GTPase-activating protein selectively expressed in the brain and found at higher concentrations, specifically at excitatory synapses in the mammalian forebrain. SynGAP has a PH domain, a C2 domain, and a highly conserved RasGAP domain, which negatively regulates both Ras activity and its downstream signaling pathways. SynGAP interacts with the PDZ domains of SAP102, as well as PSD95, a postsynaptic scaffolding protein that couples SynGAP to NMDA receptors (1). SynGAP is phosphorylated by Ca²⁺/calmodulin-dependent protein kinase II (CaMKII) at Ser765 and Ser1123, among other sites (2,3). Phosphorylation of SynGAP results in stimulation of the GTPase activity of Ras, and PSD95-dependent CaMKII phosphorylation of SynGAP increases after transient brain ischemia (1,4). SynGAP is implicated in NMDAR- and CaMKII-dependent regulation of AMPAR trafficking and plays an important role in synaptic plasticity (3,5). SynGAP is critical during neuronal development as mice lacking SynGAP protein die postnatally. Furthermore, SynGAP mutant mice have reduced long-term potentiation (LTP) and perform poorly in spatial memory tasks (6).

Database Links

UniProt ID: Q96PV0

Entrez-Gene Id: 8831

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For Research Use Only. Not for Use in Diagnostic Procedures.

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