Confocal immunofluorescent analysis of NK-92 (left, positive) and K-562 (right, negative) cells using T-bet/TBX21 (D6N8B) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) (blue pseudocolor) and β-Actin (8H10D10) Mouse mAb #3700 (red).
Flow cytometric analysis of human PBMCs using T-Bet/TBX21 (D6N8B) XP® Rabbit mAb (Alexa-Fluor® 647) co-stained with anti-CD56 (NK cells) and anti-CD19 (B cells) antibodies. T-bet/TBX21 is clearly expressed on the CD56+ NK cell population (left plot) and absent on the CD19+ B cells (right plot).
This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 647 fluorescent dye and tested in-house for direct flow cytometry analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated T-bet/TBX21 (D6N8B) XP® Rabbit mAb #13232.
Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalently purified water.
NOTE: Cells should be grown, treated, fixed and stained directly in multiwell plates, chamber slides or on coverslips.
NOTE: All subsequent incubations should be carried out at room temperature unless otherwise noted in a humid light-tight box or covered dish/plate to prevent drying and fluorochrome fading.
posted November 2006
revised December 2010
Protocol Id: 220
NOTE: Prepare solutions with RODI (reverse osmosis deionized) or equivalent grade water.
NOTE: Immunostaining of surface antigens should be performed on live cells prior to fixation/permeabilization, as per manufacturer’s requirements.
posted December 2013
Protocol Id: 626
T-Bet/TBX21 (D6N8B) XP® Rabbit mAb (Alexa Fluor ® 647 Conjugate) recognizes endogenous levels of total T-Bet/TBX21 protein.
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly465 of human T-bet/TBX21 protein.
The T-box gene family consists of transcription factors characterized by a related DNA-binding domain (T-box) of approximately 200 amino acids (1,2). The T-box genes exhibit diverse temporal and spatial patterns in the developing embryo. Studies have demonstrated members of this family play crucial roles during embryogenesis in a wide range of organisms by regulating cell fate decisions to establish the early body plan and to regulate later processes underlying organogenesis (3-5). Mutations in T-box genes are associated with many developmental defects (6). Recent studies also indicate potential roles in cancer by members of T-box family (7-9).
Explore pathways + proteins related to this product.