Render Target: STATIC
Render Timestamp: 2024-10-11T10:42:26.738Z
Commit: 56767fe525c928647c8401233a175d0d607d385d
XML generation date: 2024-09-20 06:15:11.972
Product last modified at: 2024-09-20T07:06:14.703Z
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PDP - Template Name: Monoclonal Antibody (Epitope Tag)
PDP - Template ID: *******f943b5c
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

T7-Tag (D9E1X) XP® Rabbit mAb (HRP Conjugate) #14044

Filter:
  • WB

    Supporting Data

    REACTIVITY All
    SENSITIVITY Transfected Only
    MW (kDa)
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • All-All Species Expected 

    Product Information

    Product Description

    This Cell Signaling Technology antibody is conjugated to the carbohydrate groups of horseradish peroxidase (HRP) via its amine groups. The HRP conjugated antibody is expected to exhibit the same species cross-reactivity as the unconjugated T7-Tag (D9E1X) XP® Rabbit mAb #13246.

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 136 mM NaCl, 2.6 mM KCl, 12 mM sodium phosphate (pH 7.4) dibasic, 2 mg/ml BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibodies.

    Protocol

    Specificity / Sensitivity

    T7-Tag (D9E1X) XP® Rabbit mAb (HRP Conjugate) recognizes transfected levels of recombinant protein containing the T7 epitope tag.

    Species Reactivity:

    All Species Expected

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic T7-tag peptide.

    Background

    Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.

    The T7-tag is an epitope tag composed of an 11-residue peptide encoded from the leader sequence of the T7 bacteriophage gene 10. The T7-tag has been used extensively as a general epitope tag in many expression vectors including the pET system that is based on T7 RNA polymerase expression systems (1).
    For Research Use Only. Not For Use In Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
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