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  3. TNF-α (D2D4) XP® Rabbit mAb (Mouse Specific) (PE Conjugate)
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TNF-α (D2D4) XP® Rabbit mAb (Mouse Specific) (PE Conjugate) #12779

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Inquiry Info. # 12779

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    Product Specifications

    REACTIVITY M
    SENSITIVITY Endogenous
    MW (kDa)
    Source/Isotype Rabbit
    Application Key:
    • F-Flow Cytometry 
    Species Cross-Reactivity Key:
    • M-Mouse 

    Product Information

    Product Description

    This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in mouse cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated TNF-α (D2D4) XP® Rabbit mAb (Mouse Specific) #11948.

    Product Usage Information

    Application Dilution
    Flow Cytometry (Fixed/Permeabilized) 1:50

    Storage

    Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibodies. Protect from light. Do not freeze.

    Protocol

    Specificity / Sensitivity

    TNF-α (D2D4) XP® Rabbit mAb (Mouse Specific) (PE Conjugate) recognizes endogenous levels of total TNF-α protein.

    Species Reactivity:

    Mouse

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a recombinant mouse TNF-α protein.

    Background

    TNF-α, the prototypical member of the TNF protein superfamily, is a homotrimeric type-II membrane protein (1,2). Membrane-bound TNF-α is cleaved by the metalloprotease TACE/ADAM17 to generate a soluble homotrimer (2). Both membrane and soluble forms of TNF-α are biologically active. TNF-α is produced by a variety of immune cells including T cells, B cells, NK cells, and macrophages (1). Cellular response to TNF-α is mediated through interaction with receptors TNF-R1 and TNF-R2 and results in activation of pathways that favor both cell survival and apoptosis depending on the cell type and biological context. Activation of kinase pathways (including JNK, Erk1/2, p38 MAPK, and NF-κB) promotes the survival of cells, while TNF-α-mediated activation of caspase-8 leads to programmed cell death (1,2). TNF-α plays a key regulatory role in inflammation and host defense against bacterial infection, notably Mycobacterium tuberculosis (3).

    Alternate Names

    C-domain 1; C-domain 2; Cachectin; DIF; ICD1; ICD2; Intracellular domain 1; Intracellular domain 2; MGC151434; N-terminal fragment; NTF; Tnf; TNF alpha; TNF-; TNF-a; TNF-alpha; Tnfa; TNFalpha; Tnfs; Tnfsf1a; Tnfsf2; Tnlg1f; Tumor necrosis factor; tumor necrosis factor alpha; tumor necrosis factor ligand 1f; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor-alpha; Tumor necrosis factor, membrane form; Tumor necrosis factor, soluble form

    Database Links

    UniProt ID: P06804


    Entrez-Gene Id: 21926


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