Render Target: STATIC
Render Timestamp: 2024-10-11T09:36:55.760Z
Commit: 56767fe525c928647c8401233a175d0d607d385d
XML generation date: 2024-05-06 17:08:12.416
Product last modified at: 2024-09-17T14:30:10.312Z
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PDP - Template Name: Monoclonal Antibody (Epitope Tag)
PDP - Template ID: *******f943b5c
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

V5-Tag (D3H8Q) Rabbit mAb (Magnetic Bead Conjugate) #31628

Filter:
  • IP

    Supporting Data

    REACTIVITY All
    SENSITIVITY Transfected Only
    MW (kDa)
    Source/Isotype Rabbit IgG
    Application Key:
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • All-All Species Expected 

    Product Information

    Product Description

    This Cell Signaling Technology antibody is immobilized by the covalent reaction of formylbenzamide-modified antibody with hydrazide-activated magnetic bead. V5-Tag (D3H8Q) Rabbit mAb (Magnetic Bead Conjugate) is useful for immunoprecipitation assays of V5-tagged recombinant proteins. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated V5-Tag (D3H8Q) Rabbit mAb #13202.

    Product Usage Information

    Application Dilution
    Immunoprecipitation 1:20

    Storage

    Supplied in PBS Buffer (pH 7.2), 0.1% Tween® 20. Store at 4°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    V5-Tag (D3H8Q) Rabbit mAb (Magnetic Bead Conjugate) recognizes transfected levels of recombinant protein containing the V5 epitope tag.

    Species Reactivity:

    All Species Expected

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic V5-tag peptide.

    Background

    Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.

    The V5-tag is a 14 amino acid peptide derived from a small epitope (Pk) on the P and V proteins of simian virus 5 (SV5), a member of the paramyxovirus family (1). The V5-tag can be used to detect expression of recombinant proteins in bacteria, yeast, insects, and mammalian cell systems.
    For Research Use Only. Not For Use In Diagnostic Procedures.
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