Render Target: STATIC
Render Timestamp: 2024-11-01T09:44:04.302Z
Commit: 23cb9f61fe67e1e9093fd644a533c4ff516a6463
XML generation date: 2024-09-27 20:01:12.683
Product last modified at: 2024-10-08T18:45:13.825Z
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PDP - Template Name: Buffer Kit
PDP - Template ID: *******aace572

Chaps Cell Extract Buffer (10X) #9852

    Product Information

    Reagents Not Included

    PMSF

    Product Usage Information

    Treat cells by adding fresh media containing regulator for desired time.

    Aspirate media from cultures; wash cells three times with PBS; aspirate. Scrape cells into PBS and spin down to pellet.

    Lyse cells by adding Chaps Cell Extract Buffer (1 volume of cell pellet). Resuspend cells in the buffer, freeze and thaw three times, centrifuge lysate at 14,000 rpm. Keep the supernatant and discard the pelleted cell debris.

    Add SDS Sample Buffer and heat sample to 95–100°C for 5 minutes; cool on ice.

    Microcentrifuge for 5 minutes.

    Load 5 µL onto SDS-PAGE gel (10 cm x 10 cm).

    Solutions and Reagents

    CHAPS Cell Extract Buffer (5mL) 1X concentration: 50 mM Pipes/HCl (pH 6.5), 2 mM EDTA, 0.1% Chaps, 20 µg/ml Leupeptin, 10 µg/ml Pepstatin A,10 µg/ml Aprotinin. Add DTT to 5mM.1.25 M (250X) DTT (0.25 mL).

    Storage

    Supplied as a 10X solution. Store at -20°C

    Product Description

    Chaps Cell Extract Buffer can be used to lyse cells under nondenaturing conditions and is recommended for the preparation of cytoplasmic cell lysates to be used with our caspase signaling pathway antibodies (see Companion Products).
    For Research Use Only. Not For Use In Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
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