Collagenase, Type 3 is soluble from 1-20 mg/ml with an optimal pH range of 6.3-7.5. It is recommended to reconstitute with a buffer compatible with the intended assay. Vials should be brought to room temperature prior to opening and they should not be opened in humid areas.
Collagenase, Type 3 is supplied as a lyophilized powder. This product is stable for 12 months when stored at 4ºC, protected from moisture. It is recommended to reconstitute as needed and to store solutions at a 1 mg/ml concentration at -20ºC or -80ºC. Aliquot to avoid multiple freeze/thaw cycles.
|Activity||Greater than or equal to 100 units per mg dry weight
Unit Definition: One unit releases 1 μmol of L-leucine equivalents from collagen in 5 hours at 37ºC, pH 7.5.
Collagenase, Type 3 is produced by the bacterium Clostridium histolyticum and dialyzed prior to lyophilization.
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