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XML generation date: 2024-09-19 21:57:48.057
Product last modified at: 2024-09-19T22:12:47.600Z
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Deoxyribonuclease I, Recombinant (Animal-Free, Protease and RNase Free) #53331

    Product Information

    Product Usage Information

    Deoxyribonuclease I, Recombinant (Animal-Free, Protease and RNase Free) contains 10,000 units and is sensitive to denaturation. Mix by gently inverting the tube. Do not vortex. It is recommended to reconstitute with a buffer compatible with the intended assay. Vials should be brought to room temperature prior to opening and they should not be opened in humid areas.

    Storage

    Deoxyribonuclease I, Recombinant (Animal-Free, Protease and RNase Free) is a lyophilized powder that contains glycine as a stabilizer. Store at 4ºC and protect from moisture. This product is stable for 12 months when stored at 4ºC. Once in solution, store at -20ºC. Aliquot to avoid multiple freeze/thaw cycles.

    Product Description

    Deoxyribonuclease I, Recombinant (Animal-Free, Protease and RNase Free), also known as DNase I, is isolated from bovine pancreas and produced recombinantly in Pichia pastoris. This endonuclease cleaves DNA to produce products with 5'-phospho and 3'-hydroxy ends (1). DNase I is dependent on calcium ions (Ca2+) and activated by magnesium ions (Mg2+) and manganese ions (Mn2+). The Ca2+ work to maintain the enzyme conformation and the Mg2+ and Mn2+ help to catalyze the cleavage of phosphodiester bonds (2). In the presence of Mg2+, DNase I cleaves each strand of dsDNA independently and at random. In the presence of Mn2+, DNase I cleaves each strand of dsDNA at approximately the same site. This enzyme can be used to remove genomic DNA from RNA preparations prior to RT-PCR, to degrade DNA templates after transcription reactions, and to remove unwanted DNA from samples prior to Northern blotting (3-5). DNAse is used in tissue dissociation protocols to digest any DNA that may be present due to cell damage.
    Purity > 99% purity was determined by SDS-PAGE.
    Activity Greater than or equal to 5,000 units per mg protein

    Unit Definition: One unit causes an increase in absorbance at 260 nm of 0.001 per minute at 25ºC when reacting with highly polymerized DNA at pH 5.0 (6).

    Specificity / Sensitivity

    One Kunitz unit digests 1 mg of calf thymus DNA in 10 minutes at 37ºC in 50 mM Tris, 1 mM Mg2+, 1 mM Ca2+, pH 7.8. The correlation of digestion units with Kunitz units can change in other buffer systems.

    Source / Purification

    Deoxyribonuclease I, Recombinant (Animal-Free, Protease and RNase Free) is chromatographically purified from recombinant bovine pancreatic Deoxyribonuclease I produced in Pichia pastoris. Production in yeast decreases levels of contaminating RNase and eliminates potential pathogens associated with animal-based materials.
    For Research Use Only. Not For Use In Diagnostic Procedures.
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