Deoxyribonuclease I, Recombinant (Animal-Free, Protease and RNase Free) is sensitive to denaturation. Mix by gently inverting the tube. Do not vortex. It is recommended to reconstitute with a buffer compatible with the intended assay. Vials should be brought to room temperature prior to opening and they should not be opened in humid areas.
Deoxyribonuclease I, Recombinant (Animal-Free, Protease and RNase Free) is a lyophilized powder that contains glycine as a stabilizer. Store at 4ºC and protect from moisture. This product is stable for 12 months when stored at 4ºC. Once in solution, store at -20ºC. Aliquot to avoid multiple freeze/thaw cycles.
Deoxyribonuclease I, Recombinant (Animal-Free, Protease and RNase Free), also known as DNase I, is isolated from bovine pancreas and produced recombinantly in Pichia pastoris. This endonuclease cleaves DNA to produce products with 5'-phospho and 3'-hydroxy ends (1). DNase I is dependent on calcium ions (Ca2+) and activated by magnesium ions (Mg2+) and manganese ions (Mn2+). The Ca2+ work to maintain the enzyme conformation and the Mg2+ and Mn2+ help to catalyze the cleavage of phosphodiester bonds (2). In the presence of Mg2+, DNase I cleaves each strand of dsDNA independently and at random. In the presence of Mn2+, DNase I cleaves each strand of dsDNA at approximately the same site. This enzyme can be used to remove genomic DNA from RNA preparations prior to RT-PCR, to degrade DNA templates after transcription reactions, and to remove unwanted DNA from samples prior to Northern blotting (3-5). DNAse is used in tissue dissociation protocols to digest any DNA that may be present due to cell damage.
> 99% purity was determined by SDS-PAGE.
Greater than or equal to 5,000 units per mg protein
Unit Definition: One unit causes an increase in absorbance at 260 nm of 0.001 per minute at 25ºC when reacting with highly polymerized DNA at pH 5.0 (6).
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