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4084
DRAQ5®
Buffers & Dyes

DRAQ5® #4084

APPLICATIONS

IF-IC

Confocal immunofluorescent analysis of HeLa cells using COX IV (3E11) Rabbit mAb #4850 (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5® (fluorescent DNA dye).

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Flow Cytometry

Flow cytometric analysis of Jurkat cells using Phospho-Histone H3 (Ser10) (D7N8E) XP® Rabbit mAb (PE Conjugate) #29237 versus DRAQ5® (DNA content).

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Please follow CST’s recommended IF and Flow protocols. For both applications, following secondary detection:

Flow Cytometry: Centrifuge cells and resuspend in 0.5 ml DRAQ5® diluted 1:5000 (1 µM) in Incubation Buffer. Incubate for 5 min at room temperature in the dark before analyzing cells on flow cytometer.

Immunofluorescence: Rinse samples twice in PBS for five minutes each. Dilute DRAQ5® 1:1000 (5 µM) in PBS and incubate for 5 minutes at room temperature in the dark. Rinse samples once in PBS, coat coverslips with anti-fade reagent and examine immediately using appropriate excitation wavelength.

Storage:

Store at 4°C protected from light.

DRAQ5®, 1,5-bis{[2-(di-methylamino) ethyl]amino}-4, 8-dihydroxyanthracene-9,10-dione, is a cell permeable far-red fluorescent DNA dye that can be used in live or fixed cells. This dye can be used in combination with GFP or FITC labels. DRAQ5® has been used to examine cellular DNA in flow cytometry and fluorescent microscopy applications (1-3).

  1. Smith, P.J. et al. (2000) Cytometry 40, 280-91.
  2. Wiltshire, M. et al. (2000) Cytometry 39, 217-23.
  3. Smith, P.J. et al. (1999) J Immunol Methods 229, 131-9.
For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
Alexa Fluor is a registered trademark of Life Technologies Corporation.
DRAQ5 is a registered trademark of Biostatus Limited.

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To Purchase # 4084S

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Product # Size Price
4084S
50 µl $ 204.0
4084L
200 µl $ 421.0