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DTT (Dithiothreitol)
Buffers & Dyes

DTT (Dithiothreitol) #7016

Citations (0)
Chemical structure of DTT.

Product Usage Information

DTT is supplied as a lyophilized powder. To prepare a 1 M stock solution, reconstitute the 192.8 mg supplied in the product tube in 1.12 ml ddH2O. The dissolved DTT will increase the final volume to approximately 1.25 ml.


Store lyophilized at 4ºC, desiccated. In lyophilized form, the chemical is stable for 12 months. Once in solution, store at -20ºC and use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.

Product Description

Dithiothreitol (DTT) from Cell Signaling Technology is offered in a convenient 192.8 mg lyophilized format, allowing for preparation of a fresh stock solution. This DTT reagent contains no detectable DNase or RNase activity and is suitable for use in molecular biology or protein biochemistry applications that require reduction of protein disulfide bonds.

SDS-PAGE sample buffers are routinely supplemented with 10-50 mM DTT to cleave protein disulfide bonds. Lower concentrations of DTT are routinely used to stabilize enzymes or other proteins that posses free sulfhydryl groups, which is useful in chromatin immunoprecipitation (ChIP) assays.
MW (kDa) 154.25 g/mol
Purity >99%
Molecular Formula C4H10O2S2

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not for Use in Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
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To Purchase # 7016
Cat. # Size Qty. Price
192.8 mg