Render Target: STATIC
Render Timestamp: 2024-10-11T10:49:32.702Z
Commit: 56767fe525c928647c8401233a175d0d607d385d
XML generation date: 2024-09-20 06:18:59.111
Product last modified at: 2024-09-20T07:04:23.730Z
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PDP - Template Name: Cellular Dyes
PDP - Template ID: *******0000253

ER-Tracker Green (BODIPY FL Glibenclamide) #8787

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    Product Information

    Product Usage Information

    ER-Tracker Green (BODIPY FL Glibenclamide) is supplied as a single 100 μg lyophilized vial. To make a 1 mM stock solution, reconstitute the solid in 128 μl of high quality DMSO. Dilute 1:500 directly into normal growth media for a working concentration of 2 μM and analyze immediately. Cells must be imaged live; DO NOT FIX!

    To reduce background fluorescence of the media, phenol red free media can be substituted for normal media prior to imaging.

    Storage

    Store lyophilized solid or solution at –20°C dessicated and protected from light. Once reconstituted in DMSO, the solution should be aliquoted to avoid freeze-thaw cycles. If stored properly, this reagent is stable for 6 months.

    Product Description

    ER-Tracker Green (BODIPY FL Glibenclamide) is recommended for live cell imaging only; fixation with aldehydes or alcohols will inhibit staining. Excitation: 504 nm, Emission: 511 nm, Molecular Weight: 783.10 g/mol

    Background

    ER-Tracker Green (BODIPY FL Glibenclamide) is a cell-permeant, live cell stain that is highly selective for the endoplasmic reticulum (ER). This stain consists of the green-fluorescent BODIPY FL dye and glibenclamide. Glibenclamide (glyburide) binds to sulphonylurea receptors, the regulatory subunit of ATP-sensitive K+ channels prominently expressed in the ER. The pharmacological activity of glibenclamide could potentially affect ER function. Variable expression of sulphonylurea receptors in some specialized cell types may result in non-ER labeling.
      For Research Use Only. Not For Use In Diagnostic Procedures.
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