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90232
Ghost Dye™ Red 710 Viability Dye

Ghost Dye™ Red 710 Viability Dye #90232

APPLICATIONS

Flow Cytometry

Flow cytometric analysis of live and fixed/permeabilized peripheral blood mononuclear cells, combined and stained with Ghost Dye™ Red 710 Viability Dye. Viable gate is indicated.

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Ghost Dye™ Red 710 Viability Dye is used to discriminate viable from non-viable mammalian cells in flow cytometry applications. Ghost Dye™ Red 710 Viability Dye irreversibly binds free amines available on the cell surface as well as intracellular free amines exposed in cells with compromised cell membranes. Non-viable cells with loss of membrane integrity will react with significantly more Ghost Dye™ Red 710 Viability Dye than healthy cells in the same sample. Cells that exhibit increased fluorescence intensity can be excluded from analysis.

1. Prepare the following reagents with reverse osmosis deionized (RODI) or equivalent grade water:

a. 1X PBS (azide- and protein/serum-free)

b. Incubation Buffer: Dissolve 0.5 g Bovine Serum Albumin (BSA) (#9998) in 100 ml 1X PBS. Store at 4°C.

2. Remove Ghost Dye™ from -20°C and bring to room temparature.

3. Collect cells by centrifugation and aspirate supernatant.

4. Wash cells by centrifugation in excess 1X PBS. Repeat if necessary.

5. Resuspend cells to a concentration of 1-10 x 106/mL in 1X PBS.

6. Centrifuge the Ghost Dye™ before opening then add 1 uL for each 1 mL of cell suspension and vortex immediately.

7. Incubate for 30 minutes at 4°C protected from light.

8. Wash by centrifugation in excess incubation buffer. Discard supernatant. Repeat.

9. Cells can then be stained, fixed and/or permeabilized based upon experimental design.

Ghost Dye™ Red 710 Viability Dye excited by the red (633-647 nm) laser line and has a peak emission of 710 nm

that can be detected using the recommended 710/50 band pass filter commonly used for detection of Alexa Fluor® 700.

Storage:

Store at -20°C desiccated and protected from light. This product is stable for 6 months. Aliquot to avoid excessive freeze-thaw cycles.

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
Alexa Fluor is a registered trademark of Life Technologies Corporation.
Ghost Dye is a registered trademark of Tonbo Biosciences.

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To Purchase # 90232S

Product Number Size Price
90232S 100 µl $83.00.0
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