Hyaluronidase has an optimal pH range of 4.5-6.0. It is recommended to reconstitute with a buffer compatible with the intended assay. Vials should be brought to room temperature prior to opening and they should not be opened in humid areas.
Hyaluronidase is supplied as a lyophilized powder. This product is stable for 12 months when stored at -20ºC, protected from moisture. Once in solution, aliquot to avoid multiple freeze/thaw cycles.
Hyaluronidase is a glycoprotein isolated from bovine testes that degrades hyaluronan (HA) polymers (1). This enzyme hydrolyzes the endo-N-acetylhexosaminic bonds of hyaluronic acid, chondroitinsulfuric acid A, and chondroitinsulfuric acid C to tetrasaccharide rich oligosaccharides (2). Studies have shown that hyaluronidases have the potential for clinical utility as both biomarkers and therapeutic targets when looking at a variety of cancers (1). Hyaluronidase is an effective enzyme for tissue dissociation (3,4).
Greater than or equal to 3,000 units per mg dry weight
Unit Definition: One unit is based on the change in absorbency (turbidity) at 540 nm of an internal standard assayed concurrently with each lot. Internal standard replaces USP/NF reference no longer available.
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