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SignalStain® Mounting Medium II
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SignalStain® Mounting Medium II #84583

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Product Usage Information

Important: Wear personal protective equipment. Ensure adequate ventilation. Do not handle until all safety precautions have been read and understood. Liquid and vapor is highly flammable.

Safety Information: This product contains toluene which is suspected of damaging fertility or the unborn child if inhaled. Avoid contact during pregnancy and while nursing. See Safety Data Sheet (SDS).
1. After dehydration and clearing of samples on a slide, apply a small amount of SignalStain® Mounting Medium II either on or near the section using a pipette. When the coverslip is applied, the medium will spread evenly across the coverslipped area.

2. Please allow the preparation to dry completely. Drying may take several hours when left on the lab bench, however, this process can be accelerated by incubating the slides for 15–30 min at 60ºC to 75ºC. Please ensure the slides have cooled before storage.


SignalStain® Mounting Medium II should be stored at room temperature and is stable for 12 months. Keep container tightly sealed when not in use. Slight cloudiness may appear, however, this will have no adverse effects.

Product Description

SignalStain® Mounting Medium II is for use in immunohistochemical assays. This product permanently preserves tissue or cell preparations and produces clear mounted sections, free from crystallization, with the ideal refractive index for high-resolution oil immersion microscopy.

SignalStain® Mounting Medium II retains the staining intensity of slides prepared with the following substrate kits:
SignalStain® DAB Substrate Kit #8059
SignalStain® Vibrant Red Alkaline Phosphatase Substrate Kit #76713
SignalStain® Vivid Purple Peroxidase Substrate Kit #96632
SignalStain® Deep Black Peroxidase Substrate Kit #72986

If permanent mounting of incompatible substrate kits is desired (e.g., SignalStain® Radiant Yellow Peroxidase Substrate Kit #69644 or SignalStain® Ultra Blue Alkaline Phosphatase Substrate Kit #12824), SignalStain® Mounting Prep Solution #50815 can be used after counterstaining to enable mounting with non-aqueous mounting medium, SignalStain® Mounting Medium II.

Follow the usage instructions on the appropriate substrate kit product page prior to coverslipping.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not for Use in Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
SignalStain is a registered trademark of Cell Signaling Technology, Inc.
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To Purchase # 84583
Cat. # Size Qty. Price
60 ml  (2 x 30 ml)