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PhosphoSitePlus® Resource

  • Additional protein information
  • Analytical tools


Product Description

Tris buffered saline (TBS) solution for use as the blocking buffer diluent during fluorescent western blotting. Tween 20® detergent cannot be present in the blocking buffer because it can auto-fluoresce and increase non-specific background. After the blocking step, Tween 20® can be re-introduced to subsequent diluent buffers. Chemliluminescent western blotting does not require Tween 20® omission during blocking. Product is shipped and stored at room temperature.

1X Formulation: 137 mM Sodium Chloride, 20 mM Tris. Supplied at pH 7.6.

NOTE: This product does not contain Tween 20® detergent.

Product Usage Information

Dilute Tris Buffered Saline (TBS-10X) to a 1X solution using ddH2O. This product supplies enough 10X material to make 10 liters of 1X solution.

Storage: Store 10X buffer at room temperature. At 10X, this buffer is stable for 24 months. Use within 1 week once it has been diluted to 1X.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
Tween is a registered trademark of ICI Americas, Inc.

Tris Buffered Saline (TBS-10X)