X-34 #74193
- IF
Product Information
Product Usage Information
1. X-34: Create a 5 mM stock solution of X-34 (200X) using the X-34 diluent. Create a 25 µM staining solution by further diluting the 5 mM stock solution using the X-34 diluent.
Note: X-34 diluent (40% EtOH, pH 10) is the recommended solvent for staining. A slight precipitate may be present, but will not interfere with the product's performance.
Additional Reagants (Not Supplied)
1. X-34 diluent (40% EtOH, pH 10): Combine 20 mL of EtOH in 29.5 mL of dH2O. Add 1 µL of 5M NaOH dropwise until the solution reaches pH 10. Bring the final volume of X-34 diluent to 50 mL with dH2O.
2. Differentiation buffer: 50 mM NaOH, 80% EtOH
Protocol:
1. Stain slides in 25 µM X-34 staining solution for 30 min at room temperature in the dark.
2. Rinse slides three times in dH2O.
3. Incubate for 2 min in the differentiation buffer.
4. Rinse slides three times in dH2O.
5. If desired, proceed with immunostaining.
6. Mount and image.
Storage
Fluorescent Properties
- Excitation maximum = 367 nm/Emission Maximum = 497 nm
Product Description
| Molecular Weight | 402.4 g/mol |
| Purity | ≥ 90% |
| Molecular Formula | C24H18O6 |
| CAS | 215294-98-7 |
| Solubility | Soluble in DMSO at 2 mg/mL. |
Limited Uses
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