Interested in promotions? | Click here >>
72782
7-AAD/CFSE Cell-Mediated Cytotoxicity Assay Kit
Cellular Assay Kits
Assay Kit

7-AAD/CFSE Cell-Mediated Cytotoxicity Assay Kit #72782

Reviews ()
Citations (0)
Flow Cytometry Image 1: 7-AAD/CFSE Cell-Mediated Cytotoxicity Assay Kit
Flow cytometric analysis of target K562 cells (top row) or SUP-B15 cells (bottom row) labeled with CFSE, incubated with (right column) or without (left column) effector NK-92 cells at a 12:1 effector:target cell ratio for 4 h, and stained with 7-AAD for 15 min. Cells that stain positive for both 7-AAD and CFSE represent dying target cells and are located in Q2 of the dot plot.
To Purchase # 72782S
Product # Size Price
72782S
1 Kit  (100 assays) $ 249

Product Includes Quantity (with Count) Solution Color
7-AAD Viability Dye 2 x 50 µl
CFSE Stock Solution 1 x 100 µl
Cell-Based Assay Buffer Tablet 1 x 4 ea

Protocol

PRINT

View >Collapse >

Protocol Id: 1964

Product Description

The 7-AAD/CFSE Cell-Mediated Cytotoxicity Assay Kit employs CFSE (carboxyfluorescein succinimidyl ester) to label target cells within the mixed cell population and 7-AAD (7-aminoactinomycin D) to label dead cells. This kit provides an improvement over the traditional chromium-51 (51Cr) release assay to assess cell-mediated cytotoxicity. CFSE labeling is more sensitive, does not employ radioisotopes, and cytolysis can be assessed at the single-cell level using flow cytometry.
CAS 7240-37-1, 67-68-5, 150347-59-4

Background

Cytolytic assays are routinely used to interrogate immune effector cell function. In order to interrogate immune effector cell cytolytic activity in a heterogeneous cell population of effector and target cells, it is imperative to be able to discriminate between effector and target cell populations with distinct phenotypes. As such, this kit is designed to label live target cells with the membrane permeable fluorescent dye CFSE prior to coculture with unlabelled effector cells, thus permitting a clear separation between live effector and target cells. After incubation of live effector and target cells, the DNA intercalating dye 7-AAD is added to label dying target cells with compromised plasma membranes. By using dyes with distinct spectral properties, a clear separation between four cell populations can be obtained using flow cytometry: live target cells, dead target cells, live effector cells, and dead effector cells (1).
  1. Kim, G.G. et al. (2007) 325, 51-66.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.