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53452
Cell Proliferation Tracer Kit, (Fluorometric, Blue 520)
Cellular Assay Kits
Assay Kit

Cell Proliferation Tracer Kit, (Fluorometric, Blue 520) #53452

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To Purchase # 53452S
Product # Size Price
53452S
1 Kit $ 129

Product Includes Quantity (with Count) Solution Color
Cell Proliferation Tracer Dye, Blue 520 1 x 1 
Anhydrous DMSO 1 x 150 

Product Description

The Cell Proliferation Tracer Kit (Fluorometric, Blue 520) contains Cell Proliferation Tracer Dye, Blue 520 that diffuses passively into live cells and is used for long-term cell labeling. This dye is initially a non-fluorescent ester, but is converted to a fluorescent dye by intracellular esterases. The dye then covalently reacts with amine groups on proteins, forming fluorescent conjugates that are retained in the cell. Immediately after staining, a single, bright fluorescent population will be detected by flow cytometry. Each cell division that occurs after labeling results in the appearance of a dimmer fluorescent peak on a flow cytometry histogram. The Cell Proliferation Tracer Kit (Fluorometric, Blue 520) can be used to track cell divisions in vivo or in vitro. Staining can withstand fixation and permeabilization for subsequent immunostaining.

Background

Due to their inherent metabolic stability once inside a cell, fluorescent proliferation dyes partition in an equal manner between daughter cells during the M phase of the cell cycle. This allows the principle of dye dilution to be leveraged as a means to trace multiple rounds of cell proliferation using flow cytometry. Added benefits of proliferation dyes are that they are non-radioactive and do not require cells to be actively synthesizing DNA for efficient uptake (1-3).
  1. Wallace, P.K. et al. (2008) Cytometry A 73, 1019-34.
  2. Quah, B.J. and Parish, C.R. (2012) J Immunol Methods 379, 1-14.
  3. Tario, J.D. et al. (2011) Methods Mol Biol 699, 119-64.

Limited Uses

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Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
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