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To Purchase # 13859S

13859S 1 Kit (200 assays, 96 well format) $299.00


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Product Includes Quantity Storage Temp
Reduced Glutathione Standard 1 ea -20°C
Glutathione-S-Transferase 1 ea -20°C
Tris Assay Buffer 25 ml 4°C
Digitonin Lysis Buffer 11 ml 4°C
Monochlorobimane 1 ea -20°C

Product Description

The Cellular Glutathione Detection Assay Kit employs the cell permeable dye monochlorobimane (MCB) to detect reduced glutathione (GSH) in cellular assays. MCB displays a high affinity for reduced glutathione and exhibits a very low fluorescent yield when free in solution. Upon binding to GSH, the dye exhibits a strong blue fluorescence that can be measured at an excitation wavelength of 380 nm and an emission wavelength of 460 nm. Fluorescent intensity correlates with sample GSH level. This kit can be used to either label cells directly or to detect GSH level in cell extracts. The assay can be easily applied in high throughput plate-format, flow cytometry, or fluorescent imaging.

Specificity / Sensitivity

The Cellular Glutathione Detection Assay Kit is expected to detect the reduced glutathione in cellular assays across all species.

Species Reactivity: All Species Expected
Species predicted to react based on 100% sequence homology: All Species Expected

The antioxidant glutathione is found in both reduced and oxidized states in cells. Reduced glutathione can play an important role in preventing cellular damage caused by reactive oxygen species, including free radicals and peroxides. Reduced glutathione (GSH) acts as an electron donor in the presence of free radicals and peroxides to become oxidized (GSSG). GSH also participates in redox signaling through the removal of the cellular second messenger H2O2 (1,2). Diminished glutathione levels are observed during the aging process and in oxidative stress-related diseases. The depletion of GSH is necessary for the progression of apoptosis that is mediated by various signaling pathways (3,4). Intracellular GSH levels can be a very useful indicator for overall cell health, proliferation, and death (2).

1.  Dickinson, D.A. and Forman, H.J. (2002) Ann N Y Acad Sci 973, 488-504.

2.  Pompella, A. et al. (2003) Biochem Pharmacol 66, 1499-503.

3.  Franco, R. et al. (2007) J Biol Chem 282, 30452-65.

4.  Macho, A. et al. (1997) J Immunol 158, 4612-9.

Data Sheets & Documentation

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cellular Glutathione Detection Assay Kit