News from the Bench

Discover what’s going on at CST, receive our latest application notes and tips, read our science features, and learn about our products.


Antibody Guarantee

CST Antibody Performance Guarantee


To Purchase # 12776S

12776S 1 Kit (500 assays) $129.00


Find answers on our FAQs page.


PhosphoSitePlus® Resource

  • Additional protein information
  • Analytical tools


Product Includes Quantity Storage Temp
Malachite Green Reagent 28 ml RT
Phosphate Standard (1 mM) 0.5 ml RT

Product Description

The Malachite Green Phosphate Detection Kit is a convenient and sensitive, single-step free-phosphate determination kit that can be used for measuring phosphate released during enzymatic phosphatase assays.

Specificity / Sensitivity

The Malachite Green Phosphate Detection Kit detects only free-phosphate in sample. Figure 1 demonstrates a linear range of 50 to 1600 pmol of phosphate.

The Malachite Green Phosphate Detection Kit is designed for the in vitro measurement of phosphatase activity. Protein phosphatase enzymatic activity results in the release into solution of free inorganic phosphate from protein-phosphatase substrates. This free inorganic phosphate then forms a green molybdophosphoric acid complex. Formation of this complex is monitored and quantified by measuring the absorbance at 630 nm. This absorbance is proportional to free-inorganic phosphate in solution and correlates with the activity of phosphatase (1,2).

1.  Baykov, A.A. et al. (1988) Anal Biochem 171, 266-70.

2.  Geladopoulos, T.P. et al. (1991) Anal Biochem 192, 112-6.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Malachite Green Phosphate Detection Kit