|Product Includes||Quantity (with Count)||Storage Temp|
|Malachite Green Reagent||2 x 28 ml||RT|
|Phosphate Standard (1 mM)||1 x 0.5 ml||RT|
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
|Volume (µl) of diluted stock (0.1mM)||160||140||120||100||80||60||40||20||10||5||0|
|Volume (µl) of distilled water or diluent||90||110||130||150||170||190||210||230||240||245||250|
|Phosphate [pmol] per 25 µl||1600||1400||1200||1000||800||600||400||200||100||50||0|
NOTE: Malachite Green is a highly sensitive phosphate detection solution. Make sure any reagent used in the assay is free of inorganic phosphates, as this will greatly increase the background absorbance of the assay. Enzyme reactions should be performed in a final volume of 25 µl (the same volume as that used for the standard curve). Before proceeding, confirm that your enzyme samples are free of contaminating inorganic phosphate. To determine phosphate contamination, add Malachite Green Reagent (100 µl) to 2-5 µl of sample. Any phosphate contamination will result in a color change to green. Measure absorbance at 620-660 nm and compare to standard curve to determine amount of contaminating inorganic phosphate. If required, contaminating inorganic phosphate can be removed using a desalting column or dialysis.
posted June 2020
Protocol Id: 2058
Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.
Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.