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Serine/Threonine Kinase Substrate Screening Kit

Serine/Threonine Kinase Substrate Screening Kit #7400

Inquiry Info. # 7400

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Product Information

Product Description

The Serine/Threonine Kinase Substrate Screening Kit provides 87 biotinylated nonphospho-peptides as kinase substrates. Also included are biotinylated phospho and nonphospho-peptides of identical sequence as positive and negative controls respectively. Potential substrate phosphorylation is detected by the corresponding phospho-specific antibodies.
The paired phospho-specific antibody and peptide substrate can be used for high throughput peptide based kinase screens (e.g. FP, TRF, AlphaScreen).

Specificity / Sensitivity

10 to 1000+ fold signal-to-noise ratio of phospho- versus nonphospho-peptides measured by DELFIA^® assay.

Background

In order to expedite the isolation of substrates for novel kinases, Cell Signaling Technology has developed a kinase/substrate development kit. Numerous serine/threonine kinases have become pharmaceutically important, including GSK, Akt and p38. With the cloning of hundreds of uncharacterized serine/threonine kinases, drug discovery researchers are seeking to quickly find substrates for high throughput screening programs.
With a total of 87 different peptide/phospho-specific antibody pairs on the array, a very diverse group of substrates is represented. Signal-to-noise ratios have been optimized and range from 10 to over 1000. The phospho-specific antibody and peptide substrate pair can be used for subsequent high throughput peptide based kinase screens (e.g., FP, TRF, AlphaScreen).

Alternate Names

assay development; HTS; screening; screeningkit; substrate; substrate identification

Limited Uses

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For Research Use Only. Not for Use in Diagnostic Procedures.

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